CD63 Antibody (E-12) recommended for detection of CD63 of human origin by WB, IP, IF, IHC(P) and ELISA

CD63 Antibody (E-12): sc-365604

CD63 Antibody (E-12) is rated 4.0 out of 5 by 9.
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Datasheets

    • CD63 Antibody (E-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 45-238 mapping at the C-terminus of CD63 of human origin
    • CD63 Antibody (E-12) is recommended for detection of CD63 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-CD63 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD63 (E-12): sc-365604.

Does this antibody come with a biotin conjugate?

Asked by: Jugal
Thank you for your question. We also offer this antibody in biotin-conjugated form. Please contact our technical service directly so we can send you a quotation. We also offer biotin-labeled secondary binding proteins. You can find m-IgGκ BP-B: sc-516142 for kappa-Ig here: https://www.scbt.com/scbt/de/product/m-igg-kappa-bp-b
Answered by: SCBT Heidelberg Support
Date published: 2019-01-12

Does this antibody come conjugated with biotin?

Asked by: Jsuthar
Thank you for your question. Please contact our technical service if you require the biotinylated version of this antibody.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using a directly conjugated mouse monoclonal primary antibody, a different catalog number SC-5275. This antibody is currently available conjugated to phycoerythrin (sc-5275 PE) or fluoroscein (sc-5275 FITC), Alexa Fluor® 488 (sc-5275 AF488) or Alexa Fluor® 647 (sc-5275 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 12
Date published: 2017-01-10
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Rated 4 out of 5 by from クリアなバンド データシートと同様の位置にクリアなバンドを確認することができて満足しています。特異性も十分だと思います。
Date published: 2019-08-09
Rated 1 out of 5 by from Does not work for WB in MCF7 cell extracts We bought this antibody to detect the exosomal marker CD63 but didn't get any specific signal with it, only background (even using Femto reagent). an anti-CD9 (C-4) western blot performed in parallel with the same extracts gave a good signal in comparison.
Date published: 2019-01-31
Rated 2 out of 5 by from Cd63 I ordered this antibody as a sample,but did not have enough success with it. I was not able to see a visible band for my exosome samples including my positive controls. Need to check how it performs with my other samples.
Date published: 2018-01-20
Rated 5 out of 5 by from Excellent Antibody I received it recently. Very good immunoblot bands obtained with this antibody. I rate this antibody high. Thanks to Santa Cruz
Date published: 2018-01-11
Rated 4 out of 5 by from Used for WB A little background, but no non-specific band, 1:800 been diluted,no prolbem happend.
Date published: 2017-06-25
Rated 5 out of 5 by from Strong signal Worked well for western blotting with human stomach tissue, the results provide a specific signal.
Date published: 2017-02-07
Rated 5 out of 5 by from A perfect choice for immunostaining! This antibody will give you a great signal in your immunostaining. It will provide you with bright and specific signal. A perfect choice.
Date published: 2017-01-10
Rated 5 out of 5 by from Outstanding Outstanding ihc and western blot results;pleased with product
Date published: 2017-01-05
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