CD55 Antibody (H-7): sc-133220

CD55 Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects CD55 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD55 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD55, also known as decay accelerating factor (DAF), is a glycosylphosphatidylinositol (GPI)-anchored single-chain glycoprotein that plays a crucial role in regulating the complement system. CD55 is primarily located on the surface of cells that are in contact with serum, including both hematopoietic and many non-hematopoietic cells. CD55 expression on these cells is vital as CD55 protects them from complement-mediated lysis by inhibiting the formation of the membrane attack complex, thereby preventing potential damage from the immune response. CD55 interacts with specific proteins such as C3b and C4b, which are key components of the complement pathway, further underscoring CD55′s importance in maintaining cellular integrity and immune homeostasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD55 Antibody (H-7) References:

1. Acquired and transient RBC CD55 deficiency (Inab phenotype) and anti-IFC.  |  Matthes, T., et al. 2002. Transfusion. 42: 1448-57. PMID: 12421218
2. The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy.  |  Mikesch, JH., et al. 2006. Cell Oncol. 28: 223-32. PMID: 17167176
3. Expression of CD55 on red blood cells of β-thalassemia patients.  |  Obaid, JM., et al. 2014. Hemoglobin. 38: 339-44. PMID: 25026028
4. Parainfluenza virus 5 upregulates CD55 expression to produce virions with enhanced resistance to complement-mediated neutralization.  |  Li, Y., et al. 2016. Virology. 497: 305-313. PMID: 27505156
5. Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor.  |  Weng, L., et al. 2016. Sci Rep. 6: 38632. PMID: 27929130
6. CD55 and CD59 expression protects HER2-overexpressing breast cancer cells from trastuzumab-induced complement-dependent cytotoxicity.  |  Wang, Y., et al. 2017. Oncol Lett. 14: 2961-2969. PMID: 28928834
7. Incorporation of CD55 into the Zika Viral Envelope Contributes to Its Stability against Human Complement.  |  Malekshahi, Z., et al. 2021. Viruses. 13: PMID: 33808725
8. Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains.  |  Wang, Q., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35046043
9. miR-132-3p regulates antibody-mediated complement-dependent cytotoxicity in colon cancer cells by directly targeting CD55.  |  Fan, Y., et al. 2023. Clin Exp Immunol. 211: 57-67. PMID: 36571232
10. CD55 Variant Associated with Pegylated-interferon α Therapy Response in HBeAg-positive Chronic Hepatitis B Patients.  |  Chen, J., et al. 2023. J Clin Transl Hepatol. 11: 295-303. PMID: 36643051

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD55 Antibody (H-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CD55 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD55 Antibody (H-7) Specifications

Alternate Product Name: CD55

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 35-353

Epitope Target: CD55

Epitope Species: human

Product Reactivity/Detection: CD55

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CD55 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD55 antibody is CD55 Antibody (NaM16-4D3): sc-51733.