Date published: 2026-5-8
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CD48 Antibody (4H9) - Western Blotting - Image 358958
CD48 Antibody (4H9): sc-8397. Western blot analysis of CD48 expression in Raji whole cell lysate.
CD48 Antibody (4H9): sc-8397. Western blot analysis of CD48 expression in Raji whole cell lysate.

CD48 Antibody (4H9): sc-8397

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Datasheets
  • CD48 Antibody (4H9) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
  • raised against stimulated human leukocytes
  • recommended for detection of CD48 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA
  • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD48 Antibody (4H9) for WB applications. These reagents are now offered in bundles with CD48 Antibody (4H9) (see ordering information below).
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CD48 Antibody (4H9) is a mouse monoclonal IgG1 antibody that detects CD48 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). CD48 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that plays a crucial role in immune responses by acting as a low-affinity receptor for CD2, facilitating interactions between T cells and antigen-presenting cells. CD48 expression is found on all peripheral blood T, B, and null cells, as well as on thymocytes, eosinophils, and certain epithelial cells, underscoring CD48′s importance in the regulation of immune cell activation and proliferation. CD48 associates with the tyrosine kinase Lck, which is essential for T cell receptor signaling, thereby influencing T cell development and function. CD48 expression on hematopoietic and endothelial cells in rats, and on thymocytes, lymph node cells, most macrophages, and bone marrow cells in mice, highlights CD48′s significance in both adaptive and innate immune responses. With broad species reactivity and versatile applications, anti-CD48 antibody (4H9) serves as an invaluable tool for researchers studying immune cell interactions and signaling pathways.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

CD48 Antibody (4H9) References:

  1. The CD48 receptor mediates Staphylococcus aureus human and murine eosinophil activation.  |  Minai-Fleminger, Y., et al. 2014. Clin Exp Allergy. 44: 1335-46. PMID: 25255823
  2. Roles of CD48 in regulating immunity and tolerance.  |  McArdel, SL., et al. 2016. Clin Immunol. 164: 10-20. PMID: 26794910
  3. CD48 is a key molecule of immunomodulation affecting prognosis in glioma.  |  Zou, C., et al. 2019. Onco Targets Ther. 12: 4181-4193. PMID: 31213836
  4. AML1-ETO inhibits acute myeloid leukemia immune escape by CD48.  |  Wang, Z., et al. 2021. Leuk Lymphoma. 62: 937-943. PMID: 33225787
  5. GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma.  |  Wang, Z., et al. 2021. J Immunother Cancer. 9: PMID: 34489334
  6. CD48-expressing non-small-cell lung cancer cells are susceptible to natural killer cell-mediated cytotoxicity.  |  Park, EJ., et al. 2022. Arch Pharm Res. 45: 1-10. PMID: 34905179
  7. Epigenetic regulation of CD38/CD48 by KDM6A mediates NK cell response in multiple myeloma.  |  Liu, J., et al. 2024. Nat Commun. 15: 1367. PMID: 38355622
  8. Suppression of adaptive NK cell expansion by macrophage-mediated phagocytosis inhibited by 2B4-CD48.  |  Li, R., et al. 2024. Cell Rep. 43: 113800. PMID: 38386559
  9. CCR7 and CD48 as Predicted Targets in Acute Rejection Related to M1 Macrophage after Pediatric Kidney Transplantation.  |  Zhang, J., et al. 2024. J Immunol Res. 2024: 6908968. PMID: 38957433
  10. Function of CD48 and its regulation by Epstein-Barr virus.  |  Thorley-Lawson, DA., et al. 1993. Biochem Soc Trans. 21: 976-80. PMID: 8132103

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CD48 Antibody (4H9)

sc-8397
200 µg/ml
$322.00

CD48 Antibody (4H9): m-IgG Fc BP-HRP Bundle

sc-536562
200 µg Ab; 10 µg BP
$361.00

CD48 Antibody (4H9): m-IgGκ BP-HRP Bundle

sc-533713
200 µg Ab; 40 µg BP
$361.00

CD48 Antibody (4H9) FITC

sc-8397 FITC
200 µg/ml
$336.00

CD48 Antibody (4H9) PE

sc-8397 PE
200 µg/ml
$349.00

Can CD48 (4H9): sc-8397 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD48 (4H9): sc-8397 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Well-rounded antibody for CD48This antibody does it all! It's good for Western blot and IF, but it's also really good for Flow cytometry. A great choice for all your CD48 detection needs!
Date published: 2017-02-03
Rated 4 out of 5 by from Good Western with heavy signal achieved usingGood Western with heavy signal achieved using human CD48 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-07-11
Rated 4 out of 5 by from Produced great immunofluorescence membraneProduced great immunofluorescence membrane staining in methanol-fixed IB4 cells. -SCBT QC
Date published: 2015-05-27
Rated 5 out of 5 by from Published good quality flow cytometry dataPublished good quality flow cytometry data in mouse NK92-C1, 293, BaF3, P815, and primary NK cells. -SCBT Publication Review
Date published: 2015-05-23
Rated 4 out of 5 by from Great FCM data of human peripheral bloodGreat FCM data of human peripheral blood leukocytes. -SCBT QC
Date published: 2015-05-10
Rated 4 out of 5 by from Robust WB band achieved using IB4 wholeRobust WB band achieved using IB4 whole cell lysate. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Publishable Western blot results using humanPublishable Western blot results using human SSChigh and CCR3+. -SCBT Publication Review
Date published: 2015-02-12
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CD48 Antibody (4H9) is rated 4.4 out of 5 by 7.
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