CD48 Antibody (5-4.8): sc-8400

CD48 Antibody (5-4.8) is a mouse monoclonal IgG2a kappa light chain antibody that detects CD48 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-CD48 antibody (5-4.8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD48 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that plays a crucial role in immune responses by acting as a low-affinity receptor for CD2, facilitating interactions between T cells and antigen-presenting cells. CD48 expression on all peripheral blood T, B, and null cells, as well as thymocytes and eosinophils, underscores CD48′s importance in immune cell signaling and activation. CD48 is found on hematopoietic and endothelial cells in rats, and in mice, CD48 is present on thymocytes, lymph node cells, most macrophages, and bone marrow cells. CD48 has been shown to associate with the tyrosine kinase Lck, which is vital for T cell receptor signaling, highlighting CD48′s significance in modulating immune responses.

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CD48 Antibody (5-4.8) References:

1. The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1.  |  Korínek, V., et al. 1991. Immunogenetics. 33: 108-12. PMID: 1999350
2. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.  |  Bazil, V., et al. 1989. Folia Biol (Praha). 35: 289-97. PMID: 2534480
3. Monoclonal antibodies against a rat leucocyte antigen block antigen-induced T-cell responses via an effect on accessory cells.  |  Arvieux, J., et al. 1986. Immunology. 58: 337-42. PMID: 2942461
4. CD48 Expression on Eosinophils in Nasal Polyps of Chronic Rhinosinusitis Patients.  |  Zoabi, Y., et al. 2021. Int Arch Allergy Immunol. 182: 962-970. PMID: 33910197
5. MRC OX-45 antigen: a leucocyte/endothelium rat membrane glycoprotein of 45,000 molecular weight.  |  Arvieux, J., et al. 1986. Mol Immunol. 23: 983-90. PMID: 3537734
6. Impact of HIV-1 Vpu-mediated downregulation of CD48 on NK-cell-mediated antibody-dependent cellular cytotoxicity.  |  Marchitto, L., et al. 2023. mBio. 14: e0078923. PMID: 37404017
7. Hu Ly-M3--a human leukocyte antigen.  |  Vaughan, HA., et al. 1983. Transplantation. 36: 446-50. PMID: 6623618
8. Increased expression of CD48 on neutrophils activated in childhood patients with aplastic anemia.  |  Katsuura, M., et al. 1994. Int J Hematol. 60: 281-5. PMID: 7534499
9. The association of the protein tyrosine kinases p56lck and p60fyn with the glycosyl phosphatidylinositol-anchored proteins Thy-1 and CD48 in rat thymocytes is dependent on the state of cellular activation.  |  Garnett, D., et al. 1993. Eur J Immunol. 23: 2540-4. PMID: 8104794
10. The structure and ligand interactions of CD2: implications for T-cell function.  |  Davis, SJ. and van der Merwe, PA. 1996. Immunol Today. 17: 177-87. PMID: 8871350
11. Both human alpha/beta and gamma interferons upregulate the expression of CD48 cell surface molecules.  |  Tissot, C., et al. 1997. J Interferon Cytokine Res. 17: 17-26. PMID: 9041467
12. Detection of a soluble form of the leukocyte surface antigen CD48 in plasma and its elevation in patients with lymphoid leukemias and arthritis.  |  Smith, GM., et al. 1997. J Clin Immunol. 17: 502-9. PMID: 9418191

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD48 Antibody (5-4.8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CD48 Antibody (5-4.8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD48 Antibody (5-4.8) Specifications

Alternate Product Name: CD48

Product Clone Name: 5-4.8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: CD48

Epitope Species: human

Product Reactivity/Detection: CD48

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CD48 Antibody (5-4.8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin