Date published: 2026-4-12

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CD47 Antibody (OX101): sc-53050

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Datasheets
  • CD47 Antibody (OX101) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
  • raised against thymocytes of rat origin
  • Anti-CD47 Antibody (OX101) is recommended for detection of CD47 of mouse and rat origin by WB, IP, IF, IHC(P) and FCM
  • Anti-CD47 Antibody (OX101) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD47 Antibody (OX101) for WB and IHC(P) applications. These reagents are now offered in bundles with CD47 Antibody (OX101) (see ordering information below).
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CD47 Antibody (OX101) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD47 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD47 Antibody (OX101) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD47 is an integral membrane protein that plays a crucial role in regulating cation fluxes across cell membranes, which is vital for maintaining cellular homeostasis and signaling. CD47 is involved in the increase of intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix, facilitating important cellular processes such as migration and proliferation. CD47 serves as a receptor for the C-terminal cell-binding domain of thrombospondin, which is significant for cell-cell interactions and tissue repair. CD47 is absent from Rh-null erythrocytes, yet plays a critical role in cell adhesion in non-erythroid cells and may help prevent premature elimination of erythrocytes. CD47 is expressed on various cell types, including hemopoietic cells, epithelial cells, endothelial cells, and fibroblasts, with particularly strong expression in brain and mesenchymal cells, highlighting CD47′s importance in both normal physiology and potential pathological conditions.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CD47 Antibody (OX101)

sc-53050
200 µg/ml
$322.00

CD47 Antibody (OX101): m-IgG Fc BP-HRP Bundle

sc-526761
200 µg Ab; 10 µg BP
$361.00

CD47 Antibody (OX101): m-IgG1 BP-HRP Bundle

sc-532134
200 µg Ab; 20 µg BP
$361.00

CD47 Antibody (OX101) AC

sc-53050 AC
500 µg/ml, 25% agarose
$424.00

CD47 Antibody (OX101) HRP

sc-53050 HRP
200 µg/ml
$322.00

CD47 Antibody (OX101) FITC

sc-53050 FITC
200 µg/ml
$336.00

CD47 Antibody (OX101) PE

sc-53050 PE
200 µg/ml
$349.00

CD47 Antibody (OX101) Alexa Fluor® 488

sc-53050 AF488
200 µg/ml
$364.00

CD47 Antibody (OX101) Alexa Fluor® 546

sc-53050 AF546
200 µg/ml
$364.00

CD47 Antibody (OX101) Alexa Fluor® 594

sc-53050 AF594
200 µg/ml
$364.00

CD47 Antibody (OX101) Alexa Fluor® 647

sc-53050 AF647
200 µg/ml
$364.00

CD47 Antibody (OX101) Alexa Fluor® 680

sc-53050 AF680
200 µg/ml
$364.00

CD47 Antibody (OX101) Alexa Fluor® 790

sc-53050 AF790
200 µg/ml
$364.00

Can CD47 (OX101): sc-53050 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD47 (OX101): sc-53050 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_4, d_9, h_7CST
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Rated 1 out of 5 by from IHC for Rat heart FFPE tissueUnfortunately did not working on Rat FFPE heart tissues for both 6.0pH and 9.0pH antigen retrieval.
Date published: 2025-03-03
Rated 5 out of 5 by from StrongStrong, robust signal in Western blot analysis of CD47 expression in rat brain and rat hypothalamus tissue extracts. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Produced nice Indirect FCM data for rat peripheralProduced nice Indirect FCM data for rat peripheral blood leukocytes stained with CD47 . -SCBT QC
Date published: 2014-12-19
Rated 5 out of 5 by from IHCP publishable data with rat flapsIHCP publishable data with rat flaps - SCBT Publication Review
Date published: 2014-12-19
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CD47 Antibody (OX101) is rated 4.0 out of 5 by 4.
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