CD40 Antibody (H-10) recommended for detection of CD40 of human origin by WB, IP, IF, IHC(P) and ELISA

CD40 Antibody (H-10): sc-13128

CD40 Antibody (H-10) is rated 3.9 out of 5 by 13.
  • y_2020, m_9, d_21, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_en_US, sid_13128, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 137ms
  • REVIEWS, PRODUCT
5
7
4
3
3
0
2
1
1
2

Datasheets

    • Anti-CD40 Antibody (H-10) is a mouse monoclonal IgG2b (kappa light chain) CD40 antibody provided at 200 µg/ml
    • raised against amino acids 74-193 of CD40 of human origin
    • CD40 Antibody (H-10) is recommended for detection of CD40 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-CD40 Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD40 (H-10): sc-13128.

Can CD40 Antibody (H-10) antibody be used in western blot or IHC of rat tissue?

Asked by: H_Raphael
Thank you for your question. We do not yet have any data or product citations using this antibody in rat samples, and so it is not a recommended species at this time. As an alternative, we would recommend using, CD40 Antibody (HM40-3): sc-20010.
Answered by: Technical Support
Date published: 2019-07-26

Can CD40 (H-10): sc-13128 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD40 (H-10): sc-13128 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2020, m_9, d_21, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_13128, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 142ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great antibody! This antibody works great on paraffin embeded tissue!
Date published: 2018-07-12
Rated 1 out of 5 by from Only detects albumin I tried this antibody for multiple samples to detect EVs. It only detects albumin and the is absolutely no band for CD40.
Date published: 2018-03-13
Rated 4 out of 5 by from stains stains some bv in PFA-fixed paraffin-embedded human retina sections
Date published: 2018-02-15
Rated 5 out of 5 by from Good results in WB It works very well in WB at 1:1000 dilution. I will always use it
Date published: 2017-07-12
Rated 2 out of 5 by from Good for cell IF staining This AB showed specific staining in cell's cytoplasm.
Date published: 2017-07-07
Rated 1 out of 5 by from does not work on macaque tissue antibody did not detect CD40 in paraffin embedded macaque retina tissue when used at a dilution of 1:50 ON.
Date published: 2016-12-20
Rated 5 out of 5 by from Published data for WB in HUVECs Published data for WB in HUVECs. -SCBT Publication Review
Date published: 2015-06-22
Rated 5 out of 5 by from WB positive with ECV WB positive with ECV-304 cells. -SCBT Publication Review
Date published: 2015-04-17
  • y_2020, m_9, d_21, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_en_US, sid_13128, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.