CD40 Antibody (BE-1) recommended for detection of CD40 of human origin by WB, IP, IF and FCM

CD40 Antibody (BE-1): sc-65263

CD40 Antibody (BE-1) is rated 4.0 out of 5 by 1.
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Datasheets

    • CD40 Antibody (BE-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against L cells transfectants expressing CD40 of human origin
    • recommended for detection of CD40 of human origin by WB, IP, IF and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD40 (H-10): sc-13128 for CD40 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What blocking solution should I use with CD40 (BE-1): sc-65263 monoclonal antibody for Western blot?

Asked by: Professor Griffin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-12
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Rated 4 out of 5 by from Large WB band observed using BJAB whole cell Large WB band observed using BJAB whole cell lysate under non-reducing conditions. -SCBT QC
Date published: 2015-02-16
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