CD4 Antibody (W3/25) recommended for detection of CD4 of mouse and rat origin by IF, IHC(P) and FCM

CD4 Antibody (W3/25): sc-20079

CD4 Antibody (W3/25) is rated 4.0 out of 5 by 1.
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Datasheets

    • CD4 Antibody (W3/25) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against rat thymocyte membrane
    • recommended for detection of CD4 of mouse and rat origin by IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can CD4 (W3/25): sc-20079 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD4 (W3/25): sc-20079 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Produced great FCM data for rat peripheral Produced great FCM data for rat peripheral blood leukocytes. -SCBT QC
Date published: 2014-10-19
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