CD4 Antibody (GK1.5): sc-13573

CD4 Antibody (GK1.5) is a rat monoclonal IgG2b antibody designed to recognize the extracellular region of the CD4 protein, also known as T4 or Leu 3, across mouse, rat, and human species. Raised against CTL clone V4 cells of mouse origin, anti-CD4 antibody (GK1.5) is recommended for use in immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM), ensuring precise detection and analysis of CD4 in various experimental settings. CD4 is a membrane-bound glycoprotein composed of four extracellular immunoglobulin-like domains, playing a crucial role in the immune system by acting as a co-receptor for the T cell receptor (TCR). This interaction enhances the binding affinity between TCRs and class II major histocompatibility complex (MHC) molecules on antigen-presenting cells, which is essential for effective T cell activation and the subsequent immune response. CD4 interacts with several key proteins, including the tyrosine kinase Lck, which associates with its cytoplasmic tail to initiate downstream signaling pathways necessary for T cell activation. Additionally, CD4 forms complexes with other co-receptors such as CD3 and CD28, facilitating signal transduction and T cell co-stimulation. The ability of CD4 to interact with these proteins underscores its importance in both adaptive immunity and the pathogenesis of diseases like HIV, where CD4 serves as a primary receptor for viral entry. Beyond T helper cells, CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells, and dendritic cells, highlighting its broad role in immune regulation. CD4 monoclonal antibody (GK1.5) is an indispensable tool for researchers studying immune function, T cell biology, autoimmune diseases, and infectious diseases, providing reliable detection of CD4-positive cells and supporting advancements in immunological research and therapeutic development.

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CD4 Antibody (GK1.5) References:

1. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation.  |  Janeway, CA. 1992. Annu Rev Immunol. 10: 645-74. PMID: 1534242
2. The C-terminal region of cis-retinol/androgen dehydrogenase 1 (CRAD1) confers ER localization and in vivo enzymatic function.  |  Lidén, M., et al. 2005. Exp Cell Res. 311: 205-17. PMID: 16223484
3. Novel anti-CD4 monoclonal antibodies separate human immunodeficiency virus infection and fusion of CD4+ cells from virus binding.  |  Healey, D., et al. 1990. J Exp Med. 172: 1233-42. PMID: 1698911
4. The immunobiology of T cells with invariant gamma delta antigen receptors.  |  Allison, JP. and Havran, WL. 1991. Annu Rev Immunol. 9: 679-705. PMID: 1832874
5. Cytolytic CD4(+) T cells in viral immunity.  |  Soghoian, DZ. and Streeck, H. 2010. Expert Rev Vaccines. 9: 1453-63. PMID: 21105780
6. Identification of the residues in human CD4 critical for the binding of HIV.  |  Arthos, J., et al. 1989. Cell. 57: 469-81. PMID: 2541915
7. The Effect of Lipid Metabolism on CD4+ T Cells.  |  Cai, F., et al. 2021. Mediators Inflamm. 2021: 6634532. PMID: 33505215
8. Structure and expression of the human and mouse T4 genes.  |  Maddon, PJ., et al. 1987. Proc Natl Acad Sci U S A. 84: 9155-9. PMID: 3501122
9. Mutual regulation of CD4+ T cells and intravascular fibrin in infections.  |  Mueller, TT., et al. 2024. Haematologica. 109: 2487-2499. PMID: 38572559
10. The interaction between CD4 and MHC class II molecules and its effect on T cell function.  |  Vignali, DA. 1994. Behring Inst Mitt. 133-47. PMID: 7998907
11. T cell receptor interaction with peptide/major histocompatibility complex (MHC) and superantigen/MHC ligands is dominated by antigen.  |  Ehrich, EW., et al. 1993. J Exp Med. 178: 713-22. PMID: 8393480
12. Distinct roles for CD4 and CD8 as co-receptors in antigen receptor signalling.  |  Julius, M., et al. 1993. Immunol Today. 14: 177-83. PMID: 8499078

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD4 Antibody (GK1.5) has 66 citations in a variety of scientific publications.

Primary antibodies like Anti-CD4 Antibody (GK1.5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD4 Antibody (GK1.5) Specifications

Alternate Product Name: CD4 molecule

Product Clone Name: GK1.5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: CD4

Epitope Species: mouse

Product Reactivity/Detection: an extracellular region of CD4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 66 citations in various scientific publications

Available Conjugates/Forms for CD4 Antibody (GK1.5): AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, L: Azide-Free, PE: Phycoerythrin