CD3EAP Antibody (G-5): sc-393781

CD3EAP Antibody (G-5) is a mouse monoclonal IgG1 antibody that detects CD3EAP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD3EAP, also known as ASE1, CAST, or PAF49, is a 510 amino acid protein that plays a crucial role in the nucleus as a component of the RNA polymerase I complex, which is essential for synthesizing ribosomal RNA precursors. CD3EAP exists in two alternatively spliced isoforms, with Isoform 2 potentially participating in the T cell receptor (TCR) complex, highlighting its importance in immune response regulation. CD3EAP undergoes post-translational modifications, including tyrosine phosphorylation in response to TCR stimulation, which can influence activity and interactions within the cell. Both isoforms undergo DNA damage-dependent phosphorylation, likely mediated by ATM or ATR, underscoring CD3EAP′s involvement in cellular stress responses and significance in maintaining genomic integrity. CD3EAP monoclonal antibody (G-5) serves as an invaluable tool for researchers studying the dynamics of RNA transcription and the intricate signaling pathways involved in T cell activation and immune function.

The preferred CD3EAP antibody is CD3EAP Antibody (A-9): sc-393818.