CD3EAP Antibody (A-9): sc-393818
- CD3EAP Antibody (A-9) is a mouse monoclonal IgG2b κ CD3EAP antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 27-52 near the N-terminus of CD3EAP of human origin
- CD3EAP Antibody (A-9) is recommended for detection of CD3EAP of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and bovine and porcine
- TransCruz reagent for ChIP application (sc-393818 X, 200 µg/0.1 ml)
- Anti-CD3EAP Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for CD3EAP Antibody (A-9) for WB applications. This reagent is now offered in a bundle with CD3EAP Antibody (A-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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CD3EAP Antibody (A-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects CD3EAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CD3EAP antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD3EAP protein, also known as ASE1, CAST, or PAF49, is a 510 amino acid protein that plays a crucial role in the nucleus as a component of the RNA polymerase I (Pol I) complex, which is responsible for synthesizing ribosomal RNA precursors. CD3EAP protein is essential for ribosome biogenesis, a process vital for protein synthesis in all eukaryotic cells. CD3EAP exists in two alternatively spliced isoforms, with Isoform 2 potentially serving as a component of the T cell receptor (TCR) complex and being subject to tyrosine phosphorylation upon TCR stimulation. Both isoforms are also involved in DNA damage response mechanisms, undergoing phosphorylation likely mediated by ATM or ATR kinases, which highlights their importance in maintaining genomic stability. CD3EAP′s ability to facilitate the transcription of DNA into RNA underscores its significance in cellular function and proliferation, making CD3EAP (A-9) monoclonal antibody a valuable tool for researchers studying gene expression and T cell signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD3EAP Antibody (A-9) References:
- CAST, a novel CD3epsilon-binding protein transducing activation signal for interleukin-2 production in T cells. | Yamazaki, T., et al. 1999. J Biol Chem. 274: 18173-80. PMID: 10373416
- Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription. | Yamamoto, K., et al. 2004. Mol Cell Biol. 24: 6338-49. PMID: 15226435
- Effect of polymorphisms in XPD, RAI, ASE-1 and ERCC1 on the risk of basal cell carcinoma among Caucasians after age 50. | Vogel, U., et al. 2005. Cancer Detect Prev. 29: 209-14. PMID: 15936590
- Gene-environment interactions between smoking and a haplotype of RAI, ASE-1 and ERCC1 polymorphisms among women in relation to risk of lung cancer in a population-based study. | Vogel, U., et al. 2007. Cancer Lett. 247: 159-65. PMID: 16690207
- RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor. | Panov, KI., et al. 2006. Mol Cell Biol. 26: 5436-48. PMID: 16809778
- A haplotype of polymorphisms in ASE-1, RAI and ERCC1 and the effects of tobacco smoking and alcohol consumption on risk of colorectal cancer: a Danish prospective case-cohort study. | Hansen, RD., et al. 2008. BMC Cancer. 8: 54. PMID: 18289367
- ASE-1: a novel protein of the fibrillar centres of the nucleolus and nucleolus organizer region of mitotic chromosomes. | Whitehead, CM., et al. 1997. Chromosoma. 106: 493-502. PMID: 9426281
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD3EAP Antibody (A-9) | sc-393818 | 200 µg/ml | $322.00 | |||
CD3EAP Antibody (A-9): m-IgGκ BP-HRP Bundle | sc-524101 | 200 µg Ab, 40 µg BP | $361.00 | |||
CD3EAP Antibody (A-9) X | sc-393818 X | 200 µg/0.1 ml | $322.00 | |||
CD3EAP Antibody (A-9) AC | sc-393818 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD3EAP Antibody (A-9) HRP | sc-393818 HRP | 200 µg/ml | $322.00 | |||
CD3EAP Antibody (A-9) FITC | sc-393818 FITC | 200 µg/ml | $336.00 | |||
CD3EAP Antibody (A-9) PE | sc-393818 PE | 200 µg/ml | $349.00 | |||
CD3EAP Antibody (A-9) Alexa Fluor® 488 | sc-393818 AF488 | 200 µg/ml | $364.00 | |||
CD3EAP Antibody (A-9) Alexa Fluor® 546 | sc-393818 AF546 | 200 µg/ml | $364.00 | |||
CD3EAP Antibody (A-9) Alexa Fluor® 594 | sc-393818 AF594 | 200 µg/ml | $364.00 | |||
CD3EAP Antibody (A-9) Alexa Fluor® 647 | sc-393818 AF647 | 200 µg/ml | $364.00 | |||
CD3EAP Antibody (A-9) Alexa Fluor® 680 | sc-393818 AF680 | 200 µg/ml | $364.00 | |||
CD3EAP Antibody (A-9) Alexa Fluor® 790 | sc-393818 AF790 | 200 µg/ml | $364.00 | |||
CD3EAP (A-9) Neutralizing Peptide | sc-393818 P | 100 µg/0.5 ml | $69.00 |