CD3EAP Antibody (B-2): sc-398494

CD3EAP Antibody (B-2) is a mouse monoclonal IgG2a antibody that detects CD3EAP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD3EAP, also known as ASE1, CAST, or PAF49, is a 510 amino acid protein that plays a crucial role in the transcription process as a component of the RNA polymerase I complex, which is responsible for synthesizing ribosomal RNA precursors. CD3EAP is primarily localized in the nucleus, where CD3EAP facilitates the conversion of nucleoside triphosphates into diphosphates, effectively transcribing DNA into RNA. Notably, CD3EAP exists in two alternatively spliced isoforms, with Isoform 2 potentially serving as a component of the T cell receptor complex and being subject to tyrosine phosphorylation upon T cell receptor stimulation. Both isoforms are also involved in DNA damage response mechanisms, undergoing phosphorylation likely mediated by ATM or ATR kinases, which underscores their importance in cellular stress responses and the maintenance of genomic integrity. CD3EAP monoclonal antibody (B-2) is an essential tool for researchers studying the dynamics of RNA transcription and the intricate signaling pathways involved in T cell activation and response.

The preferred CD3EAP antibody is CD3EAP Antibody (A-9): sc-393818.