CD3EAP Antibody (A-3): sc-393301

CD3EAP Antibody (A-3) is a mouse monoclonal IgG2a antibody that detects CD3EAP in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD3EAP, also known as ASE1, CAST, or PAF49, is a 510 amino acid protein that plays a crucial role in the transcription of DNA into RNA as a component of the RNA polymerase I complex, specifically synthesizing ribosomal RNA precursors. CD3EAP is localized in the nucleus and belongs to the eukaryotic RPA34 RNA polymerase subunit family, existing in two alternatively spliced isoforms. Notably, isoform 2 of CD3EAP may participate in the T cell receptor (TCR) complex and is subject to tyrosine phosphorylation upon TCR stimulation, which is essential for T cell activation and immune response. Furthermore, both isoforms of CD3EAP undergo DNA damage-dependent phosphorylation, likely mediated by ATM or ATR, highlighting CD3EAP′s importance in cellular responses to DNA damage and maintaining genomic integrity. Anti-CD3EAP antibody (A-3) is an invaluable tool for researchers studying the mechanisms of transcription regulation and T cell signaling pathways in mouse models.

The preferred CD3EAP antibody is CD3EAP Antibody (A-9): sc-393818.