CD32-A/B/C Antibody (B-4): sc-166711

CD32-A/B/C Antibody (B-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects CD32-A, CD32-B, and CD32-C proteins of human origin, as well as Fc γ RIIb and Fc γ RIII proteins of mouse and rat origin, by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CD32-A/B/C (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD32, also known as Fc gamma RII, serves as a low-affinity receptor for the Fc fragment of aggregated immunoglobulin G (IgG), playing a crucial role in immune response by mediating immune complex clearance through macrophages and regulating antibody production by B cells. CD32 can noncooperatively bind to one or two highly glycosylated molecules, delivering a negative signal that modulates B cell activity. CD32 is expressed in several isoforms generated by alternative splicing of three distinct genes—A, B, and C—resulting in isoforms designated FcgRIIA, FcgRIIB1, FcgRIIB3, and FcgRIIC. These isoforms are found on various immune cells, including monocytes, placental trophoblasts, endothelial cells, and B lymphocytes, with specific forms present on neutrophils, highlighting CD32′s importance in both innate and adaptive immunity.

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CD32-A/B/C Antibody (B-4) References:

1. Human intravenous immunoglobulin (hIVIG) inhibits anti-CD32 antibody binding to canine DH82 cells and canine monocytes in vitro.  |  Sibley, TA., et al. 2013. Vet Immunol Immunopathol. 151: 229-34. PMID: 23276750
2. CD32 Expression of Different Memory T Cell Subpopulations in the Blood and Lymph Nodal Tissue of HIV Patients and Healthy Controls Correlates With Immune Activation.  |  Wittner, M., et al. 2018. J Acquir Immune Defic Syndr. 77: 345-349. PMID: 29293157
3. CD32 is expressed on cells with transcriptionally active HIV but does not enrich for HIV DNA in resting T cells.  |  Abdel-Mohsen, M., et al. 2018. Sci Transl Med. 10: PMID: 29669853
4. Conflicting evidence for HIV enrichment in CD32+ CD4 T cells.  |  Pérez, L., et al. 2018. Nature. 561: E9-E16. PMID: 30232423
5. CD32 Expression is not Associated to HIV-DNA content in CD4 cell subsets of individuals with Different Levels of HIV Control.  |  García, M., et al. 2018. Sci Rep. 8: 15541. PMID: 30341387
6. Important roles of CD32 in promoting suppression of IL-4 induced immune responses by a novel anti-IL-4Rα therapeutic antibody.  |  Zhao, J., et al. 2019. MAbs. 11: 837-847. PMID: 30950681
7. CD32 expressing doublets in HIV-infected gut-associated lymphoid tissue are associated with a T follicular helper cell phenotype.  |  Thornhill, JP., et al. 2019. Mucosal Immunol. 12: 1212-1219. PMID: 31239514
8. FcγRII (CD32) modulates antibody clearance in NOD SCID mice leading to impaired antibody-mediated tumor cell deletion.  |  Oldham, RJ., et al. 2020. J Immunother Cancer. 8: PMID: 32554613
9. FCGR2A-HH Gene Variants Encoding the Fc Gamma Receptor for the C-Reactive Protein Are Associated with Enhanced Monocyte CD32 Expression and Cardiovascular Events' Recurrence after Primary Acute Coronary Syndrome.  |  Paul, P., et al. 2022. Biomedicines. 10: PMID: 35203703
10. Anti-Vα24Jα18 TCR Antibody Tunes iNKT Cell Responses to Target and Kill CD1d-negative Tumors in an FcγRII (CD32)-dependent Manner.  |  Takami, M., et al. 2024. Cancer Res Commun. 4: 446-459. PMID: 38319156

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD32-A/B/C Antibody (B-4) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-CD32-A/B/C Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD32-A/B/C Antibody (B-4) Specifications

Alternate Product Name: CD32

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-206

Epitope Target: CD32

Epitope Species: human

Product Reactivity/Detection: CD32-A, CD32-B and CD32-C of human origin and Fc γ RIIb and Fc γ RIII of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for CD32-A/B/C Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD32-B antibody is CD32-B Antibody (F-4): sc-365864.