CD3-ζ Antibody (H146-968): sc-101411

CD3-ζ Antibody (H146-968) is a hamster monoclonal IgG2 antibody that detects CD3-ζ in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). CD3-ζ plays a crucial role in T cell activation as a part of the T cell receptor (TCR) complex, which is essential for the immune response. CD3-ζ is located in the plasma membrane of T cells, where CD3-ζ forms a part of the CD3 complex alongside other invariant polypeptide chains. This localization is vital because CD3-ζ transduces signals from the TCR upon antigen recognition, leading to T cell activation and proliferation. The presence of multiple immunoreceptor tyrosine-based activation motifs (ITAMs) within the CD3-ζ chain enhances CD3-ζ′s ability to recruit and activate downstream signaling molecules, such as ZAP-70, which are critical for initiating the T cell response. The unique structural features of CD3-ζ, including three ITAMs, underscore CD3-ζ′s importance in the signaling cascade that ultimately determines T cell fate and function, making CD3-ζ a key target for research in immunology and therapeutic interventions. Anti-CD3-ζ antibody (H146-968) is therefore an invaluable tool for studying T cell biology and the mechanisms underlying immune responses.

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CD3-ζ Antibody (H146-968) References:

1. Modulation of T-cell CD3-zeta chain expression during normal pregnancy.  |  Taylor, DD., et al. 2002. J Reprod Immunol. 54: 15-31. PMID: 11839393
2. Down-regulation of the CD3-zeta chain in sentinel node biopsies from breast cancer patients.  |  Schüle, J., et al. 2002. Breast Cancer Res Treat. 74: 33-40. PMID: 12150450
3. Phosphorylation of multiple CD3 zeta tyrosine residues leads to formation of pp21 in vitro and in vivo. Structural changes upon T cell receptor stimulation.  |  Koyasu, S., et al. 1992. J Biol Chem. 267: 3375-81. PMID: 1531339
4. Porcine TCR CD3zeta-chain and eta-chain.  |  Yamamoto, R., et al. 2005. Mol Immunol. 42: 1485-93. PMID: 15950741
5. Differential signal transduction via T-cell receptor CD3 zeta 2, CD3 zeta-eta, and CD3 eta 2 isoforms.  |  Bauer, A., et al. 1991. Proc Natl Acad Sci U S A. 88: 3842-6. PMID: 1708889
6. Arginase activity and CD3ζ expression after major surgery.  |  Palomero Rodríguez, MÁ., et al. 2015. Intensive Care Med. 41: 939-40. PMID: 25739821
7. MiR-214 regulates CD3ζ expression in T cells.  |  Xiao, Y., et al. 2019. Cent Eur J Immunol. 44: 127-131. PMID: 31530981
8. Alternatively Spliced Variants of Murine CD247 Influence T Cell Development and Activation, Revealing the Importance of the CD3ζ C-Terminal Region.  |  Jin, Y., et al. 2024. J Immunol. 212: 541-550. PMID: 38117282
9. CD3ζ-Mediated Signaling Protects Retinal Ganglion Cells in Glutamate Excitotoxicity of the Retina.  |  Du, R., et al. 2024. Cells. 13: PMID: 38920637
10. Reconstitution of CD3 zeta coupling to calcium mobilization via genetic complementation.  |  Donnadieu, E., et al. 1994. J Biol Chem. 269: 32828-34. PMID: 7806507

The preferred CD3-ζ antibody is CD3-ζ Antibody (6B10.2): sc-1239.