CD3-ε Antibody (UCH-T1): sc-1179

CD3-ε Antibody (UCH-T1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the CD3-epsilon protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-CD3-ε antibody (UCH-T1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The CD3-epsilon protein plays a crucial role in the immune response as a component of the T cell receptor (TCR) complex, which is essential for T cell activation and signaling. Located on the surface of T cells, CD3-epsilon is part of a multisubunit complex that transmits signals upon recognition of foreign antigens, leading to T cell activation and proliferation. This process is vital for the adaptive immune response, allowing the body to effectively respond to pathogens. The CD3 complex consists of several invariant polypeptide chains, including CD3-γ, CD3-δ, and CD3-ζ, which work together to facilitate the signaling cascade initiated by TCR engagement. Notably, the presence of immunoreceptor tyrosine-based activation motifs (ITAMs) within these chains is critical for recruiting signaling molecules, such as ZAP-70, which further propagate the activation signal. Understanding the function and importance of CD3-epsilon is essential for developing therapeutic strategies targeting T cell responses in various diseases, including cancer and autoimmune disorders.

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CD3-ε Antibody (UCH-T1) References:

1. The CD3 epsilon subunit of the TCR contains endocytosis signals.  |  Borroto, A., et al. 1999. J Immunol. 163: 25-31. PMID: 10384095
2. Structure, assembly and intracellular transport of the T cell receptor for antigen.  |  Exley, M., et al. 1991. Semin Immunol. 3: 283-97. PMID: 1686832
3. Signal transduction by the T cell antigen receptor.  |  Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
4. In vitro preparation and characterization of the human CD3 epsilon epsilon homodimer and CD3 epsilon gamma and CD3 epsilon delta heterodimers.  |  Su, Z., et al. 2009. Int J Mol Med. 24: 437-44. PMID: 19724882
5. Signal transduction. Zapping tandem SH2 domains.  |  Weiss, A. 1995. Nature. 377: 17-8. PMID: 7659151
6. Molecular basis for interaction of the protein tyrosine kinase ZAP-70 with the T-cell receptor.  |  Hatada, MH., et al. 1995. Nature. 377: 32-8. PMID: 7659156
7. Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins.  |  Neumeister, EN., et al. 1995. Mol Cell Biol. 15: 3171-8. PMID: 7760813
8. Different cytoplasmic structure of the CD3 zeta family dimer modulates the activation signal and function of T cells.  |  Aoe, T., et al. 1994. Int Immunol. 6: 1671-9. PMID: 7865460
9. The role of protein tyrosine kinases and protein tyrosine phosphatases in T cell antigen receptor signal transduction.  |  Chan, AC., et al. 1994. Annu Rev Immunol. 12: 555-92. PMID: 8011291
10. Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand.  |  Ohno, H., et al. 1994. EMBO J. 13: 1157-65. PMID: 8131747
11. Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 epsilon/gamma dimers associate indistinctly with both TCR alpha and TCR beta chains. Evidence for a double TCR heterodimer model.  |  San José, E., et al. 1998. Eur J Immunol. 28: 12-21. PMID: 9485181
12. Expression of a CD3 epsilon transgene in CD3 epsilon(null) mice does not restore CD3 gamma and delta expression but efficiently rescues T cell development from a subpopulation of prothymocytes.  |  Wang, N., et al. 1998. Int Immunol. 10: 1777-88. PMID: 9885898

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD3-ε Antibody (UCH-T1) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-CD3-ε Antibody (UCH-T1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD3-ε Antibody (UCH-T1) Specifications

Alternate Product Name: CD3 epsilon

Product Clone Name: UCH-T1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD3-ε

Epitope Species: human

Product Reactivity/Detection: CD3-ε

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for CD3-ε Antibody (UCH-T1): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin