CD14 Antibody (61D3): sc-52457

CD14 Antibody (61D3) is a mouse monoclonal IgG1 kappa light chain antibody that detects human CD14, also known as monocyte differentiation antigen CD14, by immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on the surface of monocytes and macrophages, playing a crucial role in the innate immune response by serving as a co-receptor for bacterial lipopolysaccharide (LPS) detection. CD14 interacts with proteins such as LPS-binding protein (LBP), Toll-like receptor 4 (TLR4), and myeloid differentiation factor 2 (MD-2) to form a receptor complex that initiates signaling pathways leading to the secretion of cytokines and other mediators from activated immune cells. CD14 exists in both membrane-bound (mCD14) and soluble (sCD14) forms. mCD14 directly responds to LPS and facilitates protein secretion, while sCD14 enables LPS responses in cells lacking mCD14 in the presence of LBP. Anti-CD14 antibody (61D3) is available in both conjugated and non-conjugated forms, making CD14 (61D3) antibody suitable for various applications in studying immune responses and CD14-related signaling pathways.

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CD14 Antibody (61D3) References:

1. Role of CD14 in cellular recognition of bacterial lipopolysaccharides.  |  Kitchens, RL. 2000. Chem Immunol. 74: 61-82. PMID: 10608082
2. CD14-dependent clearance of apoptotic cells by human macrophages: the role of phosphatidylserine.  |  Devitt, A., et al. 2003. Cell Death Differ. 10: 371-82. PMID: 12700637
3. CD14 facilitates invasive respiratory tract infection by Streptococcus pneumoniae.  |  Dessing, MC., et al. 2007. Am J Respir Crit Care Med. 175: 604-11. PMID: 17185649
4. CD14 plays a limited role during influenza A virus infection in vivo.  |  Dessing, MC., et al. 2007. Immunol Lett. 113: 47-51. PMID: 17825924
5. CD14-/CD34+ is the founding population of umbilical cord blood-derived endothelial progenitor cells and angiogenin1 is an important factor promoting the colony formation.  |  Sudchada, S., et al. 2012. Ann Hematol. 91: 321-9. PMID: 21808991
6. CD14 influences host immune responses and alternative activation of macrophages during Schistosoma mansoni infection.  |  Tundup, S., et al. 2014. Infect Immun. 82: 3240-51. PMID: 24866794
7. CD14 Plays a Protective Role in Experimental Inflammatory Bowel Disease by Enhancing Intestinal Barrier Function.  |  Buchheister, S., et al. 2017. Am J Pathol. 187: 1106-1120. PMID: 28411859
8. Targeting CD14 on blood derived cells improves intracortical microelectrode performance.  |  Bedell, HW., et al. 2018. Biomaterials. 163: 163-173. PMID: 29471127
9. CD14 is a cell-activating receptor for bacterial peptidoglycan.  |  Gupta, D., et al. 1996. J Biol Chem. 271: 23310-6. PMID: 8798531
10. Lipopolysaccharide and peptidoglycan: CD14-dependent bacterial inducers of inflammation.  |  Rietschel, ET., et al. 1998. Microb Drug Resist. 4: 37-44. PMID: 9533723

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD14 Antibody (61D3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-CD14 Antibody (61D3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD14 Antibody (61D3) Specifications

Alternate Product Name: CD14

Product Clone Name: 61D3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD14

Epitope Species: human

Product Reactivity/Detection: CD14

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for CD14 Antibody (61D3): FITC: Fluoroscein

The preferred CD14 antibody is CD14 Antibody (UCH-M1): sc-1182.