CD14 Antibody (61D3) recommended for detection of CD14 of human origin by IP, IF and FCM

CD14 Antibody (61D3): sc-52457

CD14 Antibody (61D3) is rated 5.0 out of 5 by 3.
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Datasheets

    • CD14 Antibody (61D3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against peripheral blood monocytes obtained from a healthy donor of human origin
    • CD14 Antibody (61D3) is recommended for detection of CD14 of human origin by IP, IF and FCM
    • Anti-CD14 Antibody (61D3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP or PerCP-Cy5.5 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD14 (61D3): sc-52457.

Can CD14 (61D3): sc-52457 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD14 (61D3): sc-52457 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great for FCM The CD14 Antibody (61D3): sc-52457 works very well for Flow Cytometry applications. This antibody has also been cited several times for further verification.
Date published: 2017-02-02
Rated 5 out of 5 by from Excellent indirect FCM data of human peripheral Excellent indirect FCM data of human peripheral blood leukocytes stained with CD14 . -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Great FCM data of THP Great FCM data of THP-1 cells. -SCBT QC
Date published: 2014-05-11
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