CD100 Antibody (C-3): sc-390675
- CD100 Antibody (C-3) is a mouse monoclonal IgG2a κ CD100 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 815-845 of CD100 of human origin
- CD100 Antibody (C-3) is recommended for detection of CD100 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CD100 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD100 Antibody (C-3) for WB applications. These reagents are now offered in bundles with CD100 Antibody (C-3) (see ordering information below).
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CD100 Antibody (C-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects CD100 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD100 (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD100, also known as SEMA4D, is a transmembrane protein that plays a crucial role in the immune system, being the only semaphorin family member expressed in immune cells. CD100 is essential for T cell proliferation and B cell aggregation, as CD100 regulates B cell responses by counteracting the inhibitory signal from CD72, which typically suppresses B cell activity. When CD100 binds to CD72, CD100 induces tyrosine dephosphorylation of CD72, leading to the dissociation of SHP-1, a negative regulator of B cell signaling. This mechanism highlights CD100′s importance in modulating immune responses, particularly in T and B cell interactions. The gene encoding human CD100 is located on chromosome 9q22.2, and CD100 expression is observed in various tissues, including embryonic and adult kidney, heart, lung, and neural tissues, indicating CD100′s multifaceted role in both development and immune function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD100 Antibody (C-3) References:
- Semaphorin signaling: a little less per-plexin. | Yu, HH. and Kolodkin, AL. 1999. Neuron. 22: 11-4. PMID: 10027283
- Plexins are a large family of receptors for transmembrane, secreted, and GPI-anchored semaphorins in vertebrates. | Tamagnone, L., et al. 1999. Cell. 99: 71-80. PMID: 10520995
- Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling. | Kumanogoh, A., et al. 2000. Immunity. 13: 621-31. PMID: 11114375
- Increased surface expression of a newly identified 150-kDa dimer early after human T lymphocyte activation. | Bougeret, C., et al. 1992. J Immunol. 148: 318-23. PMID: 1530858
- Activation signals are delivered through two distinct epitopes of CD100, a unique 150 kDa human lymphocyte surface structure previously defined by BB18 mAb. | Hérold, C., et al. 1995. Int Immunol. 7: 1-8. PMID: 7718506
- Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. | Hall, KT., et al. 1996. Proc Natl Acad Sci U S A. 93: 11780-5. PMID: 8876214
- Identification of a novel transmembrane semaphorin expressed on lymphocytes. | Furuyama, T., et al. 1996. J Biol Chem. 271: 33376-81. PMID: 8969198
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD100 Antibody (C-3) | sc-390675 | 200 µg/ml | $316.00 | |||
CD100 Antibody (C-3): m-IgG Fc BP-HRP Bundle | sc-530222 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD100 Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-523631 | 200 µg Ab, 40 µg BP | $354.00 | |||
CD100 Antibody (C-3): m-IgG2a BP-HRP Bundle | sc-547460 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD100 Antibody (C-3) AC | sc-390675 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CD100 Antibody (C-3) HRP | sc-390675 HRP | 200 µg/ml | $316.00 | |||
CD100 Antibody (C-3) FITC | sc-390675 FITC | 200 µg/ml | $330.00 | |||
CD100 Antibody (C-3) PE | sc-390675 PE | 200 µg/ml | $343.00 | |||
CD100 Antibody (C-3) Alexa Fluor® 488 | sc-390675 AF488 | 200 µg/ml | $357.00 | |||
CD100 Antibody (C-3) Alexa Fluor® 546 | sc-390675 AF546 | 200 µg/ml | $357.00 | |||
CD100 Antibody (C-3) Alexa Fluor® 594 | sc-390675 AF594 | 200 µg/ml | $357.00 | |||
CD100 Antibody (C-3) Alexa Fluor® 647 | sc-390675 AF647 | 200 µg/ml | $357.00 | |||
CD100 Antibody (C-3) Alexa Fluor® 680 | sc-390675 AF680 | 200 µg/ml | $357.00 | |||
CD100 Antibody (C-3) Alexa Fluor® 790 | sc-390675 AF790 | 200 µg/ml | $357.00 | |||
CD100 (C-3) Neutralizing Peptide | sc-390675 P | 100 µg/0.5 ml | $68.00 |