CD10 Antibody (97C5) recommended for detection of CD10 of human origin by IF, IHC(P) and FCM

CD10 Antibody (97C5): sc-19993

CD10 Antibody (97C5) is rated 5.0 out of 5 by 4.
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Datasheets

    • CD10 Antibody (97C5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against BV173 Ph1-positive human leukemia
    • recommended for detection of CD10 of human origin by IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD10 (F-4): sc-46656 for CD10 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can CD10 (97C5): sc-19993 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD10 (97C5): sc-19993 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great IF images with methanol Great IF images with methanol-fixed LNCaP cells. -SCBT QC
Date published: 2015-04-05
Rated 5 out of 5 by from Produced clear immunoperoxidase cytoplasmic Produced clear immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-03-31
Rated 5 out of 5 by from Worked well for paraffin Worked well for paraffin-embedded human peripheral blood lymphocytes. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Wonderful FCM data of human peripheral blood Wonderful FCM data of human peripheral blood leukocytes. -SCBT QC
Date published: 2014-03-19
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