cathepsin W Antibody (6D238): sc-70515

Cathepsin W Antibody (6D238) is a mouse monoclonal IgG2b antibody that detects cathepsin W in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cathepsin W is a member of a unique subgroup of cathepsin proteases, specifically expressed in CD8+ T lymphocytes, and plays a crucial role in the immune response by regulating the activation and differentiation of these cells. The expression of cathepsin W is initiated during the transition of thyrocytes to CD8+ T lymphocytes, coinciding with the downregulation of CD4+ receptors in thymocytes, highlighting its importance in T cell development. Notably, cathepsin W is localized within the rough endoplasmic reticulum in transfected Cos-7 and HeLa cells, which is significant for processing and maturation. Cathepsin W features a distinctive 21 amino acid peptide insertion between the active site histidine and asparagine residues, along with an 8-amino acid carboxy-terminal extension, contributing to unique structural characteristics. Additionally, cathepsin W exhibits an extended loop structure in the beta-sheet domain and possesses an extra disulfide bond, which may influence enzymatic activity and stability. The potential for cathepsin W to exist as a dimer, with each monomer linked by a disulfide bond, further underscores complex nature and functional significance in immune regulation.

The preferred cathepsin W antibody is cathepsin W Antibody (1B1): sc-32799.