cathepsin K Antibody (E-7): sc-48353

Cathepsin K Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects cathepsin K protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-cathepsin K antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cathepsin K, a member of the cysteine protease family, plays a crucial role in bone resorption and remodeling, making cathepsin K essential for maintaining bone health and homeostasis. This enzyme is predominantly expressed in osteoclasts, cells responsible for bone degradation, and cathepsin K activity is vital for normal physiological bone turnover. Dysregulation of cathepsin K has been implicated in various bone disorders, including osteoporosis and Paget′s disease, highlighting cathepsin K′s importance as a therapeutic target. Anti-cathepsin K antibody (E-7)′s ability to detect across multiple species enhances research and clinical applications, allowing for a deeper understanding of bone metabolism and related diseases.

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cathepsin K Antibody (E-7) References:

1. Molecular cloning and expression of human alveolar macrophage cathepsin S, an elastinolytic cysteine protease.  |  Shi, GP., et al. 1992. J Biol Chem. 267: 7258-62. PMID: 1373132
2. Molecular organization of the human cathepsin D gene.  |  Redecker, B., et al. 1991. DNA Cell Biol. 10: 423-31. PMID: 2069717
3. Cathepsin K Inhibitors for Osteoporosis: Biology, Potential Clinical Utility, and Lessons Learned.  |  Drake, MT., et al. 2017. Endocr Rev. 38: 325-350. PMID: 28651365
4. Tendon-derived cathepsin K-expressing progenitor cells activate Hedgehog signaling to drive heterotopic ossification.  |  Feng, H., et al. 2020. J Clin Invest. 130: 6354-6365. PMID: 32853181
5. Molecular cloning and sequencing of cDNA for rat cathepsin L.  |  Ishidoh, K., et al. 1987. FEBS Lett. 223: 69-73. PMID: 3666143
6. Molecular cloning and sequencing of cDNA for rat cathepsin H. Homology in pro-peptide regions of cysteine proteinases.  |  Ishidoh, K., et al. 1987. FEBS Lett. 226: 33-7. PMID: 3691815
7. Cathepsin K inhibition induces Raptor destabilization and mitochondrial dysfunction via Syk/SHP2/Src/OTUB1 axis-mediated signaling.  |  Seo, SU., et al. 2023. Cell Death Dis. 14: 366. PMID: 37330581
8. Isolation and sequencing of two cDNA clones encoding rat spleen cathepsin E and analysis of the activation of purified procathepsin E.  |  Okamoto, K., et al. 1995. Arch Biochem Biophys. 322: 103-11. PMID: 7574663
9. Molecular cloning of human cathepsin O, a novel endoproteinase and homologue of rabbit OC2.  |  Shi, GP., et al. 1995. FEBS Lett. 357: 129-34. PMID: 7805878
10. Cathepsin B, a cysteine protease implicated in metastatic progression, is also expressed during regression of the rat prostate and mammary glands.  |  Guenette, RS., et al. 1994. Eur J Biochem. 226: 311-21. PMID: 8001549
11. Molecular cloning, chromosomal location, and tissue-specific expression of the murine cathepsin G gene.  |  Heusel, JW., et al. 1993. Blood. 81: 1614-23. PMID: 8453108
12. Mouse cathepsin K: cDNA cloning and predominant expression of the gene in osteoclasts, and in some hypertrophying chondrocytes during mouse development.  |  Rantakokko, J., et al. 1996. FEBS Lett. 393: 307-13. PMID: 8814310

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cathepsin K Antibody (E-7) has 165 citations in a variety of scientific publications.

Primary antibodies like Anti-cathepsin K Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cathepsin K Antibody (E-7) Specifications

Alternate Product Name: CTSK

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 191-240

Epitope Target: cathepsin K

Epitope Species: human

Product Reactivity/Detection: cathepsin K

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 165 citations in various scientific publications

Available Conjugates/Forms for cathepsin K Antibody (E-7): AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin