cathepsin B Antibody (H-5): sc-365558
- cathepsin B Antibody (H-5) is a mouse monoclonal IgG2b κ cathepsin B antibody, cited in 55 publications, provided at 200 µg/ml
- raised against amino acids 1-339 representing full length cathepsin B of human origin
- cathepsin B Antibody (H-5) is recommended for detection of cathepsin B of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-cathepsin B Antibody (H-5) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for cathepsin B Antibody (H-5) for WB applications. This reagent is now offered in a bundle with cathepsin B Antibody (H-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Cathepsin B Antibody (H-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects cathepsin B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cathepsin B antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing flexibility for different experimental needs. Cathepsin B, a member of the cysteine protease family, plays a crucial role in protein degradation and is involved in various cellular processes, including apoptosis and antigen processing. Expression in luminal epithelial cells highlights cathepsin B′s significance as a marker for secretory cell death, making cathepsin B an important target for studies related to cancer and other diseases where cell death is a critical factor. Understanding cathepsin B function is vital, as dysregulation can lead to pathological conditions, including tumor progression and metastasis. Cathepsin B′s ability to activate other proteases and involvement in extracellular matrix remodeling further underscore cathepsin B′s importance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cathepsin B Antibody (H-5) References:
- Molecular cloning and expression of human alveolar macrophage cathepsin S, an elastinolytic cysteine protease. | Shi, GP., et al. 1992. J Biol Chem. 267: 7258-62. PMID: 1373132
- Inhibitors of cathepsin B. | Frlan, R. and Gobec, S. 2006. Curr Med Chem. 13: 2309-27. PMID: 16918357
- Molecular organization of the human cathepsin D gene. | Redecker, B., et al. 1991. DNA Cell Biol. 10: 423-31. PMID: 2069717
- Cathepsin B: Active site mapping with peptidic substrates and inhibitors. | Schmitz, J., et al. 2019. Bioorg Med Chem. 27: 1-15. PMID: 30473362
- Molecular cloning and sequencing of cDNA for rat cathepsin L. | Ishidoh, K., et al. 1987. FEBS Lett. 223: 69-73. PMID: 3666143
- Molecular cloning and sequencing of cDNA for rat cathepsin H. Homology in pro-peptide regions of cysteine proteinases. | Ishidoh, K., et al. 1987. FEBS Lett. 226: 33-7. PMID: 3691815
- Cathepsin B in programmed cell death machinery: mechanisms of execution and regulatory pathways. | Xie, Z., et al. 2023. Cell Death Dis. 14: 255. PMID: 37031185
- Isolation and sequencing of two cDNA clones encoding rat spleen cathepsin E and analysis of the activation of purified procathepsin E. | Okamoto, K., et al. 1995. Arch Biochem Biophys. 322: 103-11. PMID: 7574663
- Molecular cloning of human cathepsin O, a novel endoproteinase and homologue of rabbit OC2. | Shi, GP., et al. 1995. FEBS Lett. 357: 129-34. PMID: 7805878
- Cathepsin B, a cysteine protease implicated in metastatic progression, is also expressed during regression of the rat prostate and mammary glands. | Guenette, RS., et al. 1994. Eur J Biochem. 226: 311-21. PMID: 8001549
- Molecular cloning, chromosomal location, and tissue-specific expression of the murine cathepsin G gene. | Heusel, JW., et al. 1993. Blood. 81: 1614-23. PMID: 8453108
- Mouse cathepsin K: cDNA cloning and predominant expression of the gene in osteoclasts, and in some hypertrophying chondrocytes during mouse development. | Rantakokko, J., et al. 1996. FEBS Lett. 393: 307-13. PMID: 8814310
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cathepsin B Antibody (H-5) | sc-365558 | 200 µg/ml | $316.00 | |||
cathepsin B Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-522273 | 200 µg Ab, 40 µg BP | $354.00 | |||
cathepsin B Antibody (H-5) AC | sc-365558 AC | 500 µg/ml, 25% agarose | $416.00 | |||
cathepsin B Antibody (H-5) FITC | sc-365558 FITC | 200 µg/ml | $330.00 | |||
cathepsin B Antibody (H-5) PE | sc-365558 PE | 200 µg/ml | $343.00 | |||
cathepsin B Antibody (H-5) Alexa Fluor® 488 | sc-365558 AF488 | 200 µg/ml | $357.00 | |||
cathepsin B Antibody (H-5) Alexa Fluor® 546 | sc-365558 AF546 | 200 µg/ml | $357.00 | |||
cathepsin B Antibody (H-5) Alexa Fluor® 594 | sc-365558 AF594 | 200 µg/ml | $357.00 | |||
cathepsin B Antibody (H-5) Alexa Fluor® 647 | sc-365558 AF647 | 200 µg/ml | $357.00 | |||
cathepsin B Antibody (H-5) Alexa Fluor® 680 | sc-365558 AF680 | 200 µg/ml | $357.00 | |||
cathepsin B Antibody (H-5) Alexa Fluor® 790 | sc-365558 AF790 | 200 µg/ml | $357.00 |