CaMKII gamma Antibody (8G10C1): sc-517278

CaMKIIγ Antibody (8G10C1) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect CaMKIIγ protein, also known as CAMK2G, across mouse, rat, and human species. Anti-CaMKIIγ antibody (8G10C1) is raised against a specific epitope encompassing amino acids 322-481 of human CaMKIIγ, ensuring effective recognition in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). CaMKIIγ is a critical member of the calcium/calmodulin-dependent protein kinase II family, playing a pivotal role in calcium signaling pathways that regulate essential cellular functions such as cell cycle and gene transcription. Located primarily in the cytoplasm, CaMKIIγ can translocate to different cellular compartments in response to calcium oscillations, which is crucial for interaction with diverse substrates and modulation of various cellular processes. This dynamic localization allows CaMKIIγ to effectively participate in the regulation of cellular events, including those in the biliary epithelium where multiple isoforms are present. The structural domains of CaMKIIγ, including catalytic, regulatory, and association domains, facilitate kinase activity, activation upon calcium/calmodulin binding, and assembly into multimeric holoenzymes, respectively. Understanding the precise localization and structure of CaMKIIγ is essential for elucidating its role in normal physiology and disease states. CaMKIIγ monoclonal antibody (8G10C1) is an invaluable tool for researchers seeking to investigate the complex mechanisms of calcium-dependent signaling and regulation, thereby advancing the knowledge of cellular dynamics and identifying potential therapeutic targets.

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CaMKII gamma Antibody (8G10C1) References:

1. A role for CaMKII in T cell memory.  |  Bui, JD., et al. 2000. Cell. 100: 457-67. PMID: 10693762
2. Alternative splice variant of gamma-calmodulin-dependent protein kinase II alters activation by calmodulin.  |  Kwiatkowski, AP. and McGill, JM. 2000. Arch Biochem Biophys. 378: 377-83. PMID: 10860555
3. Human calcium/calmodulin-dependent protein kinase II gamma gene (CAMK2G): cloning, genomic structure and detection of variants in subjects with type II diabetes.  |  Gloyn, AL., et al. 2002. Diabetologia. 45: 580-3. PMID: 12032636
4. Differential autophosphorylation of CaM kinase II from phasic and tonic smooth muscle tissues.  |  Lorenz, JM., et al. 2002. Am J Physiol Cell Physiol. 283: C1399-413. PMID: 12372801
5. Comparative analyses of the three-dimensional structures and enzymatic properties of alpha, beta, gamma and delta isoforms of Ca2+-calmodulin-dependent protein kinase II.  |  Gaertner, TR., et al. 2004. J Biol Chem. 279: 12484-94. PMID: 14722083
6. Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues.  |  Nghiem, P., et al. 1993. J Biol Chem. 268: 5471-9. PMID: 8449910

The preferred p-CaMKII antibody is p-CaMKII Antibody (22B1): sc-32289.