Calregulin Antibody (A-9): sc-166837
- Calregulin Antibody (A-9) is a mouse monoclonal IgG1 κ, cited in 21 publications, provided at 200 µg/ml
- raised against amino acids 248-417 of Calregulin of human origin
- Anti-Calregulin Antibody (A-9) is recommended for detection of Calregulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Calregulin Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Calregulin Antibody (A-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Calregulin Antibody (A-9) (see ordering information below).
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Calregulin Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Calregulin in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Calregulin antibody (A-9) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Calregulin, also known as calreticulin, is a calcium-binding protein predominantly located in the lumen of the endoplasmic reticulum, where Calregulin plays a crucial role in protein folding and quality control. Calregulin′s ability to bind calcium is vital for function, as Calregulin helps regulate calcium homeostasis within the cell, which is essential for various cellular processes, including signal transduction and muscle contraction. The presence of both low- and high-affinity calcium-binding sites allows Calregulin to effectively modulate calcium levels, thereby influencing the activity of other proteins and cellular pathways. Calregulin interacts with several key proteins involved in glycoprotein maturation, ensuring that only properly folded proteins are transported to their final destinations, which is critical for maintaining cellular health and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Calregulin Antibody (A-9) References:
- Calreticulin signaling in health and disease. | Wang, WA., et al. 2012. Int J Biochem Cell Biol. 44: 842-6. PMID: 22373697
- Functional roles of calreticulin in cancer biology. | Lu, YC., et al. 2015. Biomed Res Int. 2015: 526524. PMID: 25918716
- Somatic mutations of calreticulin in myeloproliferative neoplasms. | Imai, M., et al. 2017. Int J Hematol. 105: 743-747. PMID: 28470469
- Evolving Evidence of Calreticulin as a Pharmacological Target in Neurological Disorders. | Kotian, V., et al. 2019. ACS Chem Neurosci. 10: 2629-2646. PMID: 31017385
- Calreticulin mutations in myeloproliferative neoplasms. | Shide, K. 2021. Int Rev Cell Mol Biol. 365: 179-226. PMID: 34756244
- Calreticulin as a marker and therapeutic target for cancer. | Zhang, M., et al. 2023. Clin Exp Med. 23: 1393-1404. PMID: 36335525
- Effects of calreticulin mutations on cell transformation and immunity. | Desikan, H., et al. 2023. J Cell Mol Med. 27: 1032-1044. PMID: 36916035
- Calreticulin and JAK2V617F driver mutations induce distinct mitotic defects in myeloproliferative neoplasms. | Holl, K., et al. 2024. Sci Rep. 14: 2810. PMID: 38308077
- Lysosomal degradation targets mutant calreticulin and the thrombopoietin receptor in myeloproliferative neoplasms. | Kaur, A., et al. 2024. Blood Adv. 8: 3372-3387. PMID: 38640435
- Selective targeting of mutated calreticulin by the monoclonal antibody INCA033989 inhibits oncogenic function of MPN. | Reis, ES., et al. 2024. Blood. 144: 2336-2348. PMID: 39255409
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calregulin Antibody (A-9) | sc-166837 | 200 µg/ml | $322.00 | |||
Calregulin Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-529065 | 200 µg Ab; 10 µg BP | $361.00 | |||
Calregulin Antibody (A-9): m-IgGκ BP-HRP Bundle | sc-521765 | 200 µg Ab, 40 µg BP | $361.00 | |||
Calregulin Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-543298 | 200 µg Ab; 20 µg BP | $361.00 | |||
Calregulin Antibody (A-9) AC | sc-166837 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Calregulin Antibody (A-9) HRP | sc-166837 HRP | 200 µg/ml | $322.00 | |||
Calregulin Antibody (A-9) FITC | sc-166837 FITC | 200 µg/ml | $336.00 | |||
Calregulin Antibody (A-9) PE | sc-166837 PE | 200 µg/ml | $349.00 | |||
Calregulin Antibody (A-9) Alexa Fluor® 488 | sc-166837 AF488 | 200 µg/ml | $364.00 | |||
Calregulin Antibody (A-9) Alexa Fluor® 546 | sc-166837 AF546 | 200 µg/ml | $364.00 | |||
Calregulin Antibody (A-9) Alexa Fluor® 594 | sc-166837 AF594 | 200 µg/ml | $364.00 | |||
Calregulin Antibody (A-9) Alexa Fluor® 647 | sc-166837 AF647 | 200 µg/ml | $364.00 | |||
Calregulin Antibody (A-9) Alexa Fluor® 680 | sc-166837 AF680 | 200 µg/ml | $364.00 | |||
Calregulin Antibody (A-9) Alexa Fluor® 790 | sc-166837 AF790 | 200 µg/ml | $364.00 |