Calpastatin Antibody (PI-9): sc-81172
- Calpastatin Antibody (PI-9) is a mouse monoclonal IgG1 provided at 200 µg/ml
- raised against erythrocyte Calpastatin of human origin
- recommended for detection of Calpastatin of human origin by WB and IF
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Calpastatin Antibody (PI-9). This work is in progress.
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Calpastatin Antibody (PI-9) is a mouse monoclonal IgG1 light chain antibody that detects Calpastatin in human samples through applications such as western blotting (WB) and immunofluorescence (IF). Calpastatin plays a crucial role in regulating calpain activity, which is essential for various cellular processes, including cell signaling, differentiation, and apoptosis. Anti-Calpastatin antibody (PI-9) ensures that proteolytic activity is tightly controlled by inhibiting calpain, preventing unwanted degradation of vital cellular components. This regulation is particularly important in tissues undergoing rapid changes, such as during spermatogenesis, where calpain activity must be precisely modulated to facilitate proper development and function. Additionally, Calpastatin exists in two forms—tissue type and erythrocyte type—resulting from alternative splicing and proteolytic processing, which allows for a nuanced response to cellular demands. Calpastatin monoclonal antibody (PI-9) is an invaluable tool for researchers studying the intricate balance of proteolytic activity in various biological contexts, providing insights into the mechanisms underlying cellular health and disease.
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Calpastatin Antibody (PI-9) References:
- Changes in intracellular calpastatin localization are mediated by reversible phosphorylation. | Averna, M., et al. 2001. Biochem J. 354: 25-30. PMID: 11171075
- Calpastatin is associated with lymphovascular invasion in breast cancer. | Storr, SJ., et al. 2011. Breast. 20: 413-8. PMID: 21531560
- Calpastatin overexpression in the skeletal muscle of mice prevents clenbuterol-induced muscle hypertrophy and phenotypic shift. | Douillard, A., et al. 2012. Clin Exp Pharmacol Physiol. 39: 364-72. PMID: 22300302
- Over-expression of calpastatin aggravates cardiotoxicity induced by doxorubicin. | Wang, Y., et al. 2013. Cardiovasc Res. 98: 381-90. PMID: 23455548
- Calpastatin prevents NF-κB-mediated hyperactivation of macrophages and attenuates colitis. | Huang, Z., et al. 2013. J Immunol. 191: 3778-88. PMID: 23986533
- Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling. | Ye, T., et al. 2015. PLoS One. 10: e0120178. PMID: 25786109
- Unexpected role of the L-domain of calpastatin during the autoproteolytic activation of human erythrocyte calpain. | De Tullio, R., et al. 2018. Biosci Rep. 38: PMID: 29572388
- A new human calpastatin skipped of the inhibitory region protects calpain-1 from inactivation and degradation. | Sparatore, B., et al. 2019. Biochim Biophys Acta Mol Cell Res. 1866: 1260-1271. PMID: 30959065
- Osteocyte-Derived CaMKK2 Regulates Osteoclasts and Bone Mass in a Sex-Dependent Manner through Secreted Calpastatin. | Williams, JN., et al. 2023. Int J Mol Sci. 24: PMID: 36902150
- Cleavage of the calpain inhibitor, calpastatin, during apoptosis. | Pörn-Ares, MI., et al. 1998. Cell Death Differ. 5: 1028-33. PMID: 9894609
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calpastatin Antibody (PI-9) | sc-81172 | 200 µg/ml | $316.00 |