Calnexin Antibody (TO-5): sc-80645
- Calnexin Antibody (TO-5) is a mouse monoclonal IgG1 κ, cited in 18 publications, provided at 200 µg/ml
- raised against a synthetic peptide corresponding to amino acids 529-544 of Calnexin of human origin
- Calnexin Antibody (TO-5) is recommended for detection of Calnexin of human origin by WB, IP, IF, IHC(P) and FCM
- Anti-Calnexin Antibody (TO-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Calnexin Antibody (TO-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Calnexin Antibody (TO-5) (see ordering information below).
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SEE ALSO...
Calnexin Antibody (TO-5) is a mouse monoclonal IgG1 kappa light chain antibody raised against a synthetic peptide corresponding to amino acids 529-544 of human Calnexin. Calnexin, also known as CANX or IP90, is a calcium-binding chaperone protein localized in the membrane of the endoplasmic reticulum (ER). Anti-Calnexin antibody (TO-5) plays a crucial role in the ER′s quality control system by interacting with newly synthesized N-linked glycoproteins, ensuring their proper folding and assembly. Calnexin binds to monoglucosylated glycoproteins via its lectin domain, retaining them in the ER until they achieve correct conformation. Post-translational modifications of Calnexin, such as phosphorylation, are important for regulating its chaperone activity and interactions with substrate proteins. Phosphorylation can modulate its affinity for substrates and influence ER stress responses and protein homeostasis. Proper functioning of Calnexin is essential for preventing the accumulation of misfolded proteins, which can lead to diseases like neurodegeneration and diabetes. Calnexin monoclonal antibody (TO-5) reacts with human Calnexin and is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM), providing a valuable tool for researchers studying protein folding and ER quality control mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calnexin Antibody (TO-5) | sc-80645 | 200 µg/ml | $322.00 | |||
Calnexin Antibody (TO-5): m-IgG Fc BP-HRP Bundle | sc-527052 | 200 µg Ab; 10 µg BP | $361.00 | |||
Calnexin Antibody (TO-5): m-IgG1 BP-HRP Bundle | sc-532425 | 200 µg Ab; 20 µg BP | $361.00 | |||
Calnexin Antibody (TO-5) AC | sc-80645 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Calnexin Antibody (TO-5) HRP | sc-80645 HRP | 200 µg/ml | $322.00 | |||
Calnexin Antibody (TO-5) FITC | sc-80645 FITC | 200 µg/ml | $336.00 | |||
Calnexin Antibody (TO-5) PE | sc-80645 PE | 200 µg/ml | $349.00 | |||
Calnexin Antibody (TO-5) Alexa Fluor® 488 | sc-80645 AF488 | 200 µg/ml | $364.00 | |||
Calnexin Antibody (TO-5) Alexa Fluor® 546 | sc-80645 AF546 | 200 µg/ml | $364.00 | |||
Calnexin Antibody (TO-5) Alexa Fluor® 594 | sc-80645 AF594 | 200 µg/ml | $364.00 | |||
Calnexin Antibody (TO-5) Alexa Fluor® 647 | sc-80645 AF647 | 200 µg/ml | $364.00 | |||
Calnexin Antibody (TO-5) Alexa Fluor® 680 | sc-80645 AF680 | 200 µg/ml | $364.00 | |||
Calnexin Antibody (TO-5) Alexa Fluor® 790 | sc-80645 AF790 | 200 µg/ml | $364.00 |