Caldesmon Antibody (C21): sc-58700

Caldesmon Antibody (C21) is a mouse monoclonal IgG2a kappa light chain antibody that detects Caldesmon protein of mouse, rat, human, avian, and porcine origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-Caldesmon antibody (C21) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caldesmon plays a crucial role in muscle contraction and cellular motility by binding to actin filaments, which is essential for maintaining the structural integrity of the cytoskeleton and facilitating muscle function. Caldesmon exists in two main forms: muscular Caldesmon, predominantly found in smooth muscle, and non-muscular Caldesmon, which is expressed in various non-muscle tissues. The gene encoding Caldesmon undergoes alternative splicing, resulting in five isoforms, each with distinct regulatory roles. Muscular Caldesmon, also known as high molecular weight Caldesmon or H-Caldesmon, is primarily located on thin filaments in smooth muscle, while the non-muscular isoforms (2-5), referred to as low molecular weight Caldesmon or L-Caldesmon, are widely distributed in non-muscle cells. The interaction of Caldesmon with other actin-binding proteins, such as Filamin 1, Nebulin, and Villin, highlights its importance in the dynamic regulation of the cytoskeleton, making Caldesmon (C21) monoclonal antibody an invaluable tool for researchers studying muscle biology and cellular architecture.

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Caldesmon Antibody (C21) References:

1. Developmental regulation of villin gene expression in the epithelial cell lineages of mouse digestive and urogenital tracts.  |  Maunoury, R., et al. 1992. Development. 115: 717-28. PMID: 1425351
2. Caldesmon binds to smooth muscle myosin and myosin rod and crosslinks thick filaments to actin filaments.  |  Marston, S., et al. 1992. J Muscle Res Cell Motil. 13: 206-18. PMID: 1534566
3. Actin-binding and dimerization domains of HeLa cell filamin.  |  Weihing, RR. 1988. Biochemistry. 27: 1865-9. PMID: 3378035
4. The cancer-associated fibroblasts related gene CALD1 is a prognostic biomarker and correlated with immune infiltration in bladder cancer.  |  Du, Y., et al. 2021. Cancer Cell Int. 21: 283. PMID: 34051818
5. CALD1 is a prognostic biomarker and correlated with immune infiltrates in gastric cancers.  |  Liu, Y., et al. 2021. Heliyon. 7: e07257. PMID: 34189308
6. CALD1 promotes the expression of PD-L1 in bladder cancer via the JAK/STAT signaling pathway.  |  Li, C., et al. 2021. Ann Transl Med. 9: 1441. PMID: 34733993
7. MIR100HG Regulates CALD1 Gene Expression by Targeting miR-142-5p to Affect the Progression of Bladder Cancer Cells in vitro, as Revealed by Transcriptome Sequencing.  |  Zhang, S., et al. 2021. Front Mol Biosci. 8: 793493. PMID: 35127818
8. The gene expression of CALD1, CDH2, and POSTN in fibroblast are related to idiopathic pulmonary fibrosis.  |  Wu, S., et al. 2024. Front Immunol. 15: 1275064. PMID: 38370408
9. Comprehensive analysis to identify the relationship between CALD1 and immune infiltration in glioma.  |  Xia, J., et al. 2024. Transl Cancer Res. 13: 3354-3369. PMID: 39145081
10. The complete primary structure of human nebulin and its correlation to muscle structure.  |  Labeit, S. and Kolmerer, B. 1995. J Mol Biol. 248: 308-15. PMID: 7739042
11. Multiple-sited interaction of caldesmon with Ca(2+)-calmodulin.  |  Huber, PA., et al. 1996. Biochem J. 316 (Pt 2): 413-20. PMID: 8687382
12. cDNA cloning of mouse nebulin. Evidence that the nebulin-coding sequence is highly conserved among vertebrates.  |  Zhang, JQ., et al. 1996. Eur J Biochem. 239: 835-41. PMID: 8774733

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Caldesmon Antibody (C21) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Caldesmon Antibody (C21) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Caldesmon Antibody (C21) Specifications

Alternate Product Name: Caldesmon

Product Clone Name: C21

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Caldesmon

Epitope Species: chicken

Product Reactivity/Detection: H-Caldesmon and L-Caldesmon

Species Reactivity/Detection: mouse, rat, human, avian, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Caldesmon Antibody (C21): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred H-Caldesmon antibody is H-Caldesmon Antibody (h-CALD): sc-58703.