Calbindin D28K Antibody (D-4) recommended for detection of Calbindin D28K of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Calbindin D28K Antibody (D-4): sc-365360

Calbindin D28K Antibody (D-4) is rated 4.1 out of 5 by 7.
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Datasheets

    • Calbindin D28K Antibody (D-4) is a mouse monoclonal IgG1 (kappa light chain) Calbindin D28K antibody provided at 200 µg/ml
    • raised against amino acids 35-84 mapping near the N-terminus of Calbindin D28K of human origin
    • Calbindin D28K Antibody (D-4) is recommended for detection of Calbindin D28K of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine and porcine
    • Calbindin D28K Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Calbindin D28K (D-4): sc-365360.
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Can Calbindin D28K (D-4): sc-365360 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Calbindin D28K (D-4): sc-365360 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from great this antibody work for mouse tissue for western blot
Date published: 2017-09-06
Rated 4 out of 5 by from Muy buen resultado Muy buena tinción por inmunofluorescencia de células de purkinje en tejido de ratón.
Date published: 2017-07-03
Rated 4 out of 5 by from cross species I have been trying to find an antibody to go across several species, hopefully this one will help us out.
Date published: 2017-04-05
Rated 5 out of 5 by from Amazing IHC staining! Great choice for both Western blot and IHC on paraffin tissue sections. Strong and specific!
Date published: 2017-02-02
Rated 5 out of 5 by from 高效 做了小鼠嗅球的免疫组化。该抗体1:300稀释比例使用,二抗1:400室温孵育2小时,显微镜观察发现阳性明显。推荐使用,高效。
Date published: 2017-01-05
Rated 1 out of 5 by from does not work on macaque tissue antibody did not detect calbindin in paraffin embedded macaque retina tissue when used at at dilution of 1:50 ON
Date published: 2016-12-20
Rated 5 out of 5 by from Good Western blot data of Calbindin D28K Good Western blot data of Calbindin D28K expression in mouse brain tissue extract. -SCBT QC
Date published: 2014-08-07
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