CA VB Antibody (D-9): sc-393852
- CA VB Antibody (D-9) is a mouse monoclonal IgG1 κ CA VB antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 39-66 near the N-terminus of CA VB of human origin
- CA VB Antibody (D-9) is recommended for detection of CA VB of human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- Anti-CA VB Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CA VB Antibody (D-9) for WB applications. These reagents are now offered in bundles with CA VB Antibody (D-9) (see ordering information below).
CA VB Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CA VB of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CA VB (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide, playing crucial roles in numerous biological processes such as respiration, acid-base balance, and the formation of various bodily fluids. CA VB, also known as carbonate dehydratase VB, is one of the two isoforms of CA V and is primarily localized in the mitochondria, where CA VB is involved in essential metabolic processes. CA VB is predominantly expressed in the heart, pancreas, lung, placenta, kidney, and skeletal muscle, exhibiting the highest homology with CA VA, the second isoform of CA V. Unlike CA VA, CA VB is not expressed in the liver, indicating a distinct physiological role that may be critical for the metabolic functions in tissues where CA VB is present. The unique expression pattern and mitochondrial localization of CA VB underscore CA VB′s importance in cellular metabolism and highlight CA VB′s potential as a target for research into metabolic disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CA VB Antibody (D-9) References:
- Human mitochondrial carbonic anhydrase VB. cDNA cloning, mRNA expression, subcellular localization, and mapping to chromosome x. | Fujikawa-Adachi, K., et al. 1999. J Biol Chem. 274: 21228-33. PMID: 10409679
- Mitochondrial carbonic anhydrase CA VB: differences in tissue distribution and pattern of evolution from those of CA VA suggest distinct physiological roles. | Shah, GN., et al. 2000. Proc Natl Acad Sci U S A. 97: 1677-82. PMID: 10677517
- Carbonic anhydrase isozymes in the human pancreas. | Nishimori, I. and Onishi, S. 2001. Dig Liver Dis. 33: 68-74. PMID: 11303978
- Carbonic anhydrase inhibitors: clash with Ala65 as a means for designing inhibitors with low affinity for the ubiquitous isozyme II, exemplified by the crystal structure of the topiramate sulfamide analogue. | Winum, JY., et al. 2006. J Med Chem. 49: 7024-31. PMID: 17125255
- Molecular modeling study for the binding of zonisamide and topiramate to the human mitochondrial carbonic anhydrase isoform VA. | Vitale, RM., et al. 2007. Bioorg Med Chem. 15: 4152-8. PMID: 17420132
- Carbonic anhydrases as targets for medicinal chemistry. | Supuran, CT. and Scozzafava, A. 2007. Bioorg Med Chem. 15: 4336-50. PMID: 17475500
- Carbonic anhydrases as drug targets--an overview. | Supuran, CT. 2007. Curr Top Med Chem. 7: 825-33. PMID: 17504127
- Antiobesity carbonic anhydrase inhibitors. | De Simone, G. and Supuran, CT. 2007. Curr Top Med Chem. 7: 879-84. PMID: 17504132
- Carbonic anhydrase inhibitors. Interaction of the antiepileptic drug sulthiame with twelve mammalian isoforms: kinetic and X-ray crystallographic studies. | Temperini, C., et al. 2007. Bioorg Med Chem Lett. 17: 4866-72. PMID: 17588751
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CA VB Antibody (D-9) | sc-393852 | 200 µg/ml | $322.00 | |||
CA VB Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-530605 | 200 µg Ab; 10 µg BP | $361.00 | |||
CA VB Antibody (D-9): m-IgGκ BP-HRP Bundle | sc-524119 | 200 µg Ab, 40 µg BP | $361.00 | |||
CA VB Antibody (D-9): m-IgG1 BP-HRP Bundle | sc-544159 | 200 µg Ab; 20 µg BP | $361.00 | |||
CA VB Antibody (D-9) AC | sc-393852 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CA VB Antibody (D-9) HRP | sc-393852 HRP | 200 µg/ml | $322.00 | |||
CA VB Antibody (D-9) FITC | sc-393852 FITC | 200 µg/ml | $336.00 | |||
CA VB Antibody (D-9) PE | sc-393852 PE | 200 µg/ml | $349.00 | |||
CA VB Antibody (D-9) Alexa Fluor® 488 | sc-393852 AF488 | 200 µg/ml | $364.00 | |||
CA VB Antibody (D-9) Alexa Fluor® 546 | sc-393852 AF546 | 200 µg/ml | $364.00 | |||
CA VB Antibody (D-9) Alexa Fluor® 594 | sc-393852 AF594 | 200 µg/ml | $364.00 | |||
CA VB Antibody (D-9) Alexa Fluor® 647 | sc-393852 AF647 | 200 µg/ml | $364.00 | |||
CA VB Antibody (D-9) Alexa Fluor® 680 | sc-393852 AF680 | 200 µg/ml | $364.00 | |||
CA VB Antibody (D-9) Alexa Fluor® 790 | sc-393852 AF790 | 200 µg/ml | $364.00 | |||
CA VB (D-9) Neutralizing Peptide | sc-393852 P | 100 µg/0.5 ml | $69.00 |