C8γ Antibody (H-10): sc-515150
- C8γ Antibody (H-10) is a mouse monoclonal IgG2b κ C8γ antibody provided at 200 µg/ml
- raised against amino acids 138-202 mapping at the C-terminus of C8γ of human origin
- C8 gamma Antibody (H-10) is recommended for detection of C8γ of human origin by WB, IP, IF and ELISA
- Anti-C8 gamma Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for C8γ Antibody (H-10) for WB applications. These reagents are now offered in bundles with C8γ Antibody (H-10) (see ordering information below).
C8γ Antibody (H-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects C8 gamma protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). C8γ (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C8 gamma protein, also known as complement component 8 gamma polypeptide, plays a crucial role in the complement system, which is essential for the innate immune response. C8 gamma protein is one of three polypeptides that make up C8, alongside C8α and C8β, and is vital for the formation of the membrane attack complex that can lyse pathogens. C8γ consists of 202 amino acids and is classified within the lipocalin family, known for binding small hydrophobic molecules, such as retinol. Proper functioning of C8γ is critical, as deficiencies or dysfunctions can lead to increased susceptibility to infections and autoimmune diseases, highlighting its importance in maintaining immune homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
C8γ Antibody (H-10) References:
- Structural features of the ligand binding site on human complement protein C8gamma: a member of the lipocalin family. | Chiswell, B., et al. 2007. Biochim Biophys Acta. 1774: 637-44. PMID: 17452033
- The homology of complement factor C8 gamma chain and alpha-1-microglobulin. | Hunt, LT., et al. 1987. Biochem Biophys Res Commun. 149: 282-8. PMID: 2446620
- Structural homology of human complement component C8 gamma and plasma protein HC: identity of the cysteine bond pattern. | Haefliger, JA., et al. 1987. Biochem Biophys Res Commun. 149: 750-4. PMID: 2447883
- Chromosomal assignment of genes encoding the alpha, beta, and gamma subunits of human complement protein C8: identification of a close physical linkage between the alpha and the beta loci. | Kaufman, KM., et al. 1989. Genomics. 5: 475-80. PMID: 2613233
- The eighth component of human complement: evidence that it is an oligomeric serum protein assembled from products of three different genes. | Ng, SC., et al. 1987. Biochemistry. 26: 5229-33. PMID: 3676249
- Comparative mapping of lipocalin genes in human and mouse: the four genes for complement C8 gamma chain, prostaglandin-D-synthase, oncogene-24p3, and progestagen-associated endometrial protein map to HSA9 and MMU2. | Chan, P., et al. 1994. Genomics. 23: 145-50. PMID: 7829063
- The human complement C8G gene, a member of the lipocalin gene family: polymorphisms and mapping to chromosome 9q34.3. | Dewald, G., et al. 1996. Ann Hum Genet. 60: 281-91. PMID: 8865989
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C8γ Antibody (H-10) | sc-515150 | 200 µg/ml | $322.00 | |||
C8γ Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-531301 | 200 µg Ab; 10 µg BP | $361.00 | |||
C8γ Antibody (H-10): m-IgGκ BP-HRP Bundle | sc-524956 | 200 µg Ab, 40 µg BP | $361.00 | |||
C8γ Antibody (H-10): m-IgG2b BP-HRP Bundle | sc-550276 | 200 µg Ab; 10 µg BP | $361.00 | |||
C8γ Antibody (H-10) AC | sc-515150 AC | 500 µg/ml, 25% agarose | $424.00 | |||
C8γ Antibody (H-10) HRP | sc-515150 HRP | 200 µg/ml | $322.00 | |||
C8γ Antibody (H-10) FITC | sc-515150 FITC | 200 µg/ml | $336.00 | |||
C8γ Antibody (H-10) PE | sc-515150 PE | 200 µg/ml | $349.00 | |||
C8γ Antibody (H-10) Alexa Fluor® 488 | sc-515150 AF488 | 200 µg/ml | $364.00 | |||
C8γ Antibody (H-10) Alexa Fluor® 546 | sc-515150 AF546 | 200 µg/ml | $364.00 | |||
C8γ Antibody (H-10) Alexa Fluor® 594 | sc-515150 AF594 | 200 µg/ml | $364.00 | |||
C8γ Antibody (H-10) Alexa Fluor® 647 | sc-515150 AF647 | 200 µg/ml | $364.00 | |||
C8γ Antibody (H-10) Alexa Fluor® 680 | sc-515150 AF680 | 200 µg/ml | $364.00 | |||
C8γ Antibody (H-10) Alexa Fluor® 790 | sc-515150 AF790 | 200 µg/ml | $364.00 |