C5b-9 Antibody (aE11): sc-58935

C5b-9 Antibody (aE11) is a mouse monoclonal IgG2a kappa light chain antibody that detects C5b-9 in human and porcine samples through immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA) applications. Anti-C5b-9 antibody (aE11) is available in a non-conjugated form and specifically recognizes a neoepitope exposed in C9 when incorporated into the C5b-9 complex, which can exist in both membrane-bound (membrane attack complex) and fluid phase (soluble C5b-9) forms. C5b-9 monoclonal antibody (aE11) plays a crucial role in studying the complement system, an essential part of the immune response. The C5b-9 complex forms during the activation of complement proteins, including C2, C3, C4, and C5, which are released as potent anaphylatoxins that trigger pro-inflammatory responses by binding to their respective G protein-coupled receptors. C5b-9 can lead to cell lysis, yet sublytic assembly on plasma membranes can paradoxically promote cell survival and activation of the cell cycle, highlighting a dual role in immune responses. This complex is particularly significant in multiple sclerosis, where C5b-9 may exhibit both pro-inflammatory effects during the acute phase and neuroprotective properties in the chronic phase by modulating apoptotic pathways in oligodendrocytes.

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C5b-9 Antibody (aE11) References:

1. Complement C5b-9 induces receptor tyrosine kinase transactivation in glomerular epithelial cells.  |  Cybulsky, AV., et al. 1999. Am J Pathol. 155: 1701-11. PMID: 10550326
2. Inhibition of oligodendrocyte apoptosis by sublytic C5b-9 is associated with enhanced synthesis of bcl-2 and mediated by inhibition of caspase-3 activation.  |  Soane, L., et al. 1999. J Immunol. 163: 6132-8. PMID: 10570303
3. Renal C3 synthesis in idiopathic membranous nephropathy: correlation to urinary C5b-9 excretion.  |  Montinaro, V., et al. 2000. Kidney Int. 57: 137-46. PMID: 10620195
4. Immunohistochemical localization of cytokines, C5b-9 and ICAM-1 in peripheral nerve of Guillain-Barré syndrome.  |  Putzu, GA., et al. 2000. J Neurol Sci. 174: 16-21. PMID: 10704976
5. C5b-9 terminal complement complex protects oligodendrocytes from death by regulating Bad through phosphatidylinositol 3-kinase/Akt pathway.  |  Soane, L., et al. 2001. J Immunol. 167: 2305-11. PMID: 11490019
6. Sublytic C5b-9-stimulated Schwann cell survival through PI 3-kinase-mediated phosphorylation of BAD.  |  Hila, S., et al. 2001. Glia. 36: 58-67. PMID: 11571784
7. C5b-9 complement complex in autoimmune demyelination and multiple sclerosis: dual role in neuroinflammation and neuroprotection.  |  Rus, H., et al. 2005. Ann Med. 37: 97-104. PMID: 16026117
8. The complement C5b-9 complexes induced injury of glomerular mesangial cells in rats with Thy-1 nephritis by increasing nitric oxide synthesis.  |  Wang, Y., et al. 2006. Life Sci. 79: 182-92. PMID: 16458938
9. C5b-9 terminal complex protects oligodendrocytes from apoptotic cell death by inhibiting caspase-8 processing and up-regulating FLIP.  |  Cudrici, C., et al. 2006. J Immunol. 176: 3173-80. PMID: 16493077

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-C5b-9 Antibody (aE11) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-C5b-9 Antibody (aE11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

C5b-9 Antibody (aE11) Specifications

Alternate Product Name: Complement Component 5b-9

Product Clone Name: aE11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: C5b-9

Epitope Species: human

Product Reactivity/Detection: a neoepitope exposed in C9 when incorporated into C5b-9 in both membrane bound (MAC) and fluid phase (SC5b-9) complexes

Species Reactivity/Detection: human, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for C5b-9 Antibody (aE11): N/A