C2 Antibody (E-7): sc-373809

C2 Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects C2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-C2 antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C2 protein plays a crucial role in the complement system, which is essential for immune defense. C2 protein is involved in the classical pathway of complement activation, where cleavage into C2a and C2b fragments occurs, with C2a being responsible for binding to C3 convertase, thus facilitating pathogen opsonization and promoting inflammation. This process is vital for pathogen clearance and immune response initiation. C2 deficiency is the most prevalent deficiency in the classical complement pathway and is often associated with autoimmune diseases and increased susceptibility to bacterial infections. The N-terminal extracellular domain 1 of the complement C2 receptor inhibitory trispanning (CRIT) specifically binds to C2 and interacts with the C2a fragment, effectively blocking C2 cleavage and preventing classical pathway C3 convertase formation. This regulatory mechanism highlights C2′s importance in maintaining immune homeostasis and preventing excessive inflammation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

C2 Antibody (E-7) References:

1. Continual low-level activation of the classical complement pathway.  |  Manderson, AP., et al. 2001. J Exp Med. 194: 747-56. PMID: 11560991
2. Intravascular schistosomes and complement.  |  Skelly, PJ. 2004. Trends Parasitol. 20: 370-4. PMID: 15246320
3. Complement C2 receptor inhibitor trispanning: a novel human complement inhibitory receptor.  |  Inal, JM., et al. 2005. J Immunol. 174: 356-66. PMID: 15611259
4. [Immunologic tests: C2].  |  Kitano, E. and Kitamura, H. 2005. Nihon Rinsho. 63 Suppl 7: 59-62. PMID: 16111188
5. Variation in factor B (BF) and complement component 2 (C2) genes is associated with age-related macular degeneration.  |  Gold, B., et al. 2006. Nat Genet. 38: 458-62. PMID: 16518403
6. Mannan-binding lectin activates C3 and the alternative complement pathway without involvement of C2.  |  Selander, B., et al. 2006. J Clin Invest. 116: 1425-34. PMID: 16670774
7. Homozygosity for the IgG2 subclass allotype G2M(n) protects against severe infection in hereditary C2 deficiency.  |  Jönsson, G., et al. 2006. J Immunol. 177: 722-8. PMID: 16785571
8. Complement regulatory proteins and autoimmunity.  |  Song, WC. 2006. Autoimmunity. 39: 403-10. PMID: 16923540

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-C2 Antibody (E-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-C2 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

C2 Antibody (E-7) Specifications

Alternate Product Name: C2

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 204-503

Epitope Target: C2

Epitope Species: human

Product Reactivity/Detection: C2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for C2 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent