Date published: 2026-1-30

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c-Jun Antibody (B-1): sc-166540

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Datasheets
  • c-Jun Antibody (B-1) is a mouse monoclonal IgG1 κ, cited in 39 publications, provided at 200 µg/ml
  • raised against a peptide mapping within the N-terminus of c-Jun of mouse origin
  • c-Jun Antibody (B-1) is recommended for detection of c-Jun of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
  • TransCruz reagent for ChIP application (sc-166540 X, 200 µg/0.1 ml)
  • Anti-c-Jun Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for c-Jun Antibody (B-1) for WB and IHC(P) applications. These reagents are now offered in bundles with c-Jun Antibody (B-1) (see ordering information below).
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c-Jun Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects c-Jun in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-c-Jun antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. c-Jun (B-1) antibody recognizes a key component of the transcription factor AP-1, which regulates gene expression in response to growth factors and stress signals. c-Jun′s ability to form homo- and heterodimers with other Jun and Fos oncogene family members enables binding to the TPA-response element (TRE) with high affinity, influencing genes involved in cell proliferation and survival. c-Jun (B-1) monoclonal antibody targets a protein whose interaction with Ha-Ras enhances transcriptional activity, highlighting c-Jun′s importance in oncogenic signaling pathways. Post-translational modifications, such as phosphorylation through the cAMP-dependent protein kinase A pathway, modulate c-Jun activity, emphasizing c-Jun′s significance in cellular responses and potential as a therapeutic target in cancer research.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

c-Jun Antibody (B-1)

sc-166540
200 µg/ml
$322.00

c-Jun Antibody (B-1): m-IgG Fc BP-HRP Bundle

sc-528990
200 µg Ab; 10 µg BP
$361.00

c-Jun Antibody (B-1): m-IgGκ BP-HRP Bundle

sc-521648
200 µg Ab, 40 µg BP
$361.00

c-Jun Antibody (B-1): m-IgG1 BP-HRP Bundle

sc-543252
200 µg Ab; 20 µg BP
$361.00

c-Jun Antibody (B-1) X

sc-166540 X
200 µg/0.1 ml
$322.00

c-Jun Antibody (B-1) AC

sc-166540 AC
500 µg/ml, 25% agarose
$424.00

c-Jun Antibody (B-1) HRP

sc-166540 HRP
200 µg/ml
$322.00

c-Jun Antibody (B-1) FITC

sc-166540 FITC
200 µg/ml
$336.00

c-Jun Antibody (B-1) PE

sc-166540 PE
200 µg/ml
$349.00

c-Jun Antibody (B-1) Alexa Fluor® 488

sc-166540 AF488
200 µg/ml
$364.00

c-Jun Antibody (B-1) Alexa Fluor® 546

sc-166540 AF546
200 µg/ml
$364.00

c-Jun Antibody (B-1) Alexa Fluor® 594

sc-166540 AF594
200 µg/ml
$364.00

c-Jun Antibody (B-1) Alexa Fluor® 647

sc-166540 AF647
200 µg/ml
$364.00

c-Jun Antibody (B-1) Alexa Fluor® 680

sc-166540 AF680
200 µg/ml
$364.00

c-Jun Antibody (B-1) Alexa Fluor® 790

sc-166540 AF790
200 µg/ml
$364.00

c-Jun (B-1) Neutralizing Peptide

sc-166540 P
100 µg/0.5 ml
$69.00

Is this antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: TinTin
Thank you for your question. Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using c-Jun (G-4): sc-74543 which is available conjugated to AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647 (https://www.scbt.com/scbt/product/c-jun-antibody-g-4)
Answered by: Technical Support 13
Date published: 2017-01-20

When should the TransCruz reagent sc-166540 X be used instead of the standard form sc-166540?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 9
Date published: 2017-01-17
  • y_2026, m_1, d_23, h_8CST
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Rated 5 out of 5 by from specific stainingThis antibody recognises a dominant species of the expected molecular weight.
Date published: 2017-02-06
Rated 5 out of 5 by from Beautiful IF staining!Excellent signal seen with methanol fixed samples in IF, with clear nuclear localization.
Date published: 2017-01-18
Rated 5 out of 5 by from No signal seen in WB in mouse cell linesNo signal seen in WB in mouse cell lines at 1:200 dilution.
Date published: 2015-10-22
Rated 3 out of 5 by from Works well in WB with transfected cell lysatesWorks well in WB with transfected cell lysates -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-14
Rated 5 out of 5 by from Great results with mouse embryo samples inGreat results with mouse embryo samples in Western Blot -SCBT Publication Review
Date published: 2015-02-10
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c-Jun Antibody (B-1) is rated 4.7 out of 5 by 6.
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