c-Fos Antibody (D-1): sc-8047

c-Fos Antibody (D-1) is a mouse monoclonal IgG2b kappa light chain antibody raised against amino acids 210-335 mapping at the C-terminus of human c-Fos, detecting c-Fos of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-c-Fos antibody (D-1) is available in both conjugated and non-conjugated forms, offering versatility for various experimental applications. c-Fos is a nuclear phosphoprotein that acts as a transcriptional regulator, characterized by rapid and transient expression in response to a wide array of stimuli, including growth factors, cytokines, and stress signals. c-Fos forms heterodimers with members of the Jun family—such as c-Jun, Jun B, and Jun D, to create the Activator Protein-1 (AP-1) transcription factor complex, which binds to specific DNA elements to regulate gene expression linked to cell proliferation, differentiation, and survival. Beyond interactions with Jun proteins, c-Fos also associates with transcription factors like ATF-2 and CREB, forming leucine zipper dimers that exhibit differential specificity for AP-1 and CRE sites. c-Fos is regulated by coactivators such as CBP/p300, which modulate activity through acetylation, influencing gene transcription outcomes. c-Fos is involved in regulating cytoskeletal organization and cellular motility, highlighting diverse functions beyond transcriptional regulation. Understanding these interactions and implications is vital for studying cell signaling pathways, making c-Fos a critical marker in oncogenesis, neurobiology, and cellular stress responses.

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c-Fos Antibody (D-1) References:

1. Protein kinase D2 potentiates MEK/ERK/RSK signaling, c-Fos accumulation and DNA synthesis induced by bombesin in Swiss 3T3 cells.  |  Sinnett-Smith, J., et al. 2007. J Cell Physiol. 211: 781-90. PMID: 17226786
2. Immunohistochemical analysis of p53, cyclinD1, RB1, c-fos and N-ras gene expression in hepatocellular carcinoma in Iran.  |  Moghaddam, SJ., et al. 2007. World J Gastroenterol. 13: 588-93. PMID: 17278226
3. Isolation and characterization of fra-2, an additional member of the fos gene family.  |  Nishina, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 3619-23. PMID: 2110368
4. A direct role for c-fos in AP-1-dependent gene transcription.  |  Herrera, R., et al. 1990. Cell Growth Differ. 1: 483-90. PMID: 2126190
5. The product of a fos-related gene, fra-1, binds cooperatively to the AP-1 site with Jun: transcription factor AP-1 is comprised of multiple protein complexes.  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
6. An avian transforming retrovirus isolated from a nephroblastoma that carries the fos gene as the oncogene.  |  Nishizawa, M., et al. 1987. J Virol. 61: 3733-40. PMID: 2824811
7. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.  |  Bohmann, D., et al. 1987. Science. 238: 1386-92. PMID: 2825349
8. Chromatin association and DNA binding properties of the c-fos proto-oncogene product.  |  Renz, M., et al. 1987. Nucleic Acids Res. 15: 277-92. PMID: 3103098
9. The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose.  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
10. Virus induction of osteosarcomas in mice.  |  Finkel, MP., et al. 1966. Science. 151: 698-701. PMID: 5218049

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-c-Fos Antibody (D-1) has 104 citations in a variety of scientific publications.

Primary antibodies like Anti-c-Fos Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

c-Fos Antibody (D-1) Specifications

Alternate Product Name: c-Fos

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 210-335

Epitope Target: c-Fos

Epitope Species: human

Product Reactivity/Detection: c-Fos

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 104 citations in various scientific publications

Available Conjugates/Forms for c-Fos Antibody (D-1): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, X: TransCruz Reagent