BTC Antibody (E-12): sc-514061

BTC Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects BTC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-BTC antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, plays a crucial role in cellular signaling and growth regulation. BTC is synthesized as a large transmembrane precursor that can be proteolytically cleaved to release a soluble form or function as a membrane-anchored growth factor, facilitating juxtacrine signaling. BTC stimulates homodimerization of ErbB-1 and ErbB-4, and binds and activates various combinations of heterodimeric ErbB receptors, including the oncogenic ErbB-2/ErbB-3 complex, underscoring BTC′s significance in promoting cellular proliferation and differentiation. BTC expression in certain human malignancies suggests potential involvement in tumor growth progression, making BTC a valuable target for therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BTC Antibody (E-12) References:

1. Identification of betacellulin as a major peptide growth factor in milk: purification, characterization and molecular cloning of bovine betacellulin.  |  Dunbar, AJ., et al. 1999. Biochem J. 344 Pt 3: 713-21. PMID: 10585857
2. Auto-induction and growth stimulatory effect of betacellulin in human pancreatic cancer cells.  |  Kawaguchi, M., et al. 2000. Int J Oncol. 16: 37-41. PMID: 10601546
3. Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.  |  O-charoenrat, P., et al. 2000. Cancer Res. 60: 1121-8. PMID: 10706134
4. Analysis of coding variants in the betacellulin gene in type 2 diabetes and insulin secretion in African American subjects.  |  Elbein, SC., et al. 2006. BMC Med Genet. 7: 62. PMID: 16869959
5. Expression of betacellulin and epidermal growth factor receptor in hepatocellular carcinoma: implications for angiogenesis.  |  Moon, WS., et al. 2006. Hum Pathol. 37: 1324-32. PMID: 16949929
6. Betacellulin enhances ovarian cancer cell migration by up-regulating Connexin43 via MEK-ERK signaling.  |  Zhao, J., et al. 2020. Cell Signal. 65: 109439. PMID: 31654720
7. Betacellulin promotes tumor development and EGFR mutant lung cancer growth by stimulating the EGFR pathway and suppressing apoptosis.  |  Chava, S., et al. 2022. iScience. 25: 104211. PMID: 35494243
8. Multi-omic network analysis identified betacellulin as a novel target of omega-3 fatty acid attenuation of western diet-induced nonalcoholic steatohepatitis.  |  Padiadpu, J., et al. 2023. EMBO Mol Med. 15: e18367. PMID: 37859621
9. Cloning and expression of cDNA encoding human betacellulin, a new member of the EGF family.  |  Sasada, R., et al. 1993. Biochem Biophys Res Commun. 190: 1173-9. PMID: 8439318
10. Betacellulin: a mitogen from pancreatic beta cell tumors.  |  Shing, Y., et al. 1993. Science. 259: 1604-7. PMID: 8456283
11. Epidermal growth factor and betacellulin mediate signal transduction through co-expressed ErbB2 and ErbB3 receptors.  |  Alimandi, M., et al. 1997. EMBO J. 16: 5608-17. PMID: 9312020
12. The oncogenic ErbB-2/ErbB-3 heterodimer is a surrogate receptor of the epidermal growth factor and betacellulin.  |  Pinkas-Kramarski, R., et al. 1998. Oncogene. 16: 1249-58. PMID: 9546426

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BTC Antibody (E-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-BTC Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BTC Antibody (E-12) Specifications

Alternate Product Name: Betacellulin

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-178

Epitope Target: BTC

Epitope Species: human

Product Reactivity/Detection: BTC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for BTC Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TGFα antibody is TGFα Antibody (D-6): sc-374433.