BTBD4 Antibody (C-5): sc-390260
- BTBD4 Antibody (C-5) is a mouse monoclonal IgG1 κ BTBD4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 391-421 within an internal region of BTBD4 of human origin
- BTBD4 Antibody (C-5) is recommended for detection of BTBD4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BTBD4 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-390260 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BTBD4 Antibody (C-5) for WB applications. These reagents are now offered in bundles with BTBD4 Antibody (C-5) (see ordering information below).
BTBD4 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects BTBD4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BTBD4 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BTBD4, also known as zinc finger protein 340 (ZNF340) or zinc finger and BTB domain-containing protein 46 (ZBTB46), is a 589 amino acid protein characterized by the presence of a single BTB/POZ domain. This domain is crucial for mediating both homomeric and heteromeric interactions, which are essential for the function of transcriptional regulators involved in chromatin remodeling. The BTB/POZ domain′s ability to interact with the SMRT/N-CoR-mSin3A histone deacetylase complex highlights its role in repressing gene transcription, a function that is vital for maintaining cellular homeostasis and regulating gene expression in response to various stimuli. Notably, the deletion of this domain, as seen in the oncogenic PLZF-RAR chimera associated with promyelocytic leukemias, leads to a loss of transcriptional repression, suggesting that BTBD4 may be integral to the regulation of genes involved in cell differentiation and proliferation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BTBD4 Antibody (C-5) References:
- Recruitment of SMRT/N-CoR-mSin3A-HDAC-repressing complexes is not a general mechanism for BTB/POZ transcriptional repressors: the case of HIC-1 and gammaFBP-B. | Deltour, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 14831-6. PMID: 10611298
- Critical residues within the BTB domain of PLZF and Bcl-6 modulate interaction with corepressors. | Melnick, A., et al. 2002. Mol Cell Biol. 22: 1804-18. PMID: 11865059
- DNA sequence and analysis of human chromosome 9. | Humphray, SJ., et al. 2004. Nature. 429: 369-74. PMID: 15164053
- POZ for effect--POZ-ZF transcription factors in cancer and development. | Kelly, KF. and Daniel, JM. 2006. Trends Cell Biol. 16: 578-87. PMID: 16996269
- Histone deacetylase associated with mSin3A mediates repression by the acute promyelocytic leukemia-associated PLZF protein. | David, G., et al. 1998. Oncogene. 16: 2549-56. PMID: 9627120
- Components of the SMRT corepressor complex exhibit distinctive interactions with the POZ domain oncoproteins PLZF, PLZF-RARalpha, and BCL-6. | Wong, CW. and Privalsky, ML. 1998. J Biol Chem. 273: 27695-702. PMID: 9765306
- Crystal structure of the BTB domain from PLZF. | Ahmad, KF., et al. 1998. Proc Natl Acad Sci U S A. 95: 12123-8. PMID: 9770450
- The BCL-6 POZ domain and other POZ domains interact with the co-repressors N-CoR and SMRT. | Huynh, KD. and Bardwell, VJ. 1998. Oncogene. 17: 2473-84. PMID: 9824158
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTBD4 Antibody (C-5) | sc-390260 | 200 µg/ml | $322.00 | |||
BTBD4 Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-530112 | 200 µg Ab; 10 µg BP | $361.00 | |||
BTBD4 Antibody (C-5): m-IgGκ BP-HRP Bundle | sc-523471 | 200 µg Ab, 40 µg BP | $361.00 | |||
BTBD4 Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-543874 | 200 µg Ab; 20 µg BP | $361.00 | |||
BTBD4 Antibody (C-5) X | sc-390260 X | 200 µg/0.1 ml | $322.00 | |||
BTBD4 Antibody (C-5) AC | sc-390260 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BTBD4 Antibody (C-5) HRP | sc-390260 HRP | 200 µg/ml | $322.00 | |||
BTBD4 Antibody (C-5) FITC | sc-390260 FITC | 200 µg/ml | $336.00 | |||
BTBD4 Antibody (C-5) PE | sc-390260 PE | 200 µg/ml | $349.00 | |||
BTBD4 Antibody (C-5) Alexa Fluor® 488 | sc-390260 AF488 | 200 µg/ml | $364.00 | |||
BTBD4 Antibody (C-5) Alexa Fluor® 546 | sc-390260 AF546 | 200 µg/ml | $364.00 | |||
BTBD4 Antibody (C-5) Alexa Fluor® 594 | sc-390260 AF594 | 200 µg/ml | $364.00 | |||
BTBD4 Antibody (C-5) Alexa Fluor® 647 | sc-390260 AF647 | 200 µg/ml | $364.00 | |||
BTBD4 Antibody (C-5) Alexa Fluor® 680 | sc-390260 AF680 | 200 µg/ml | $364.00 | |||
BTBD4 Antibody (C-5) Alexa Fluor® 790 | sc-390260 AF790 | 200 µg/ml | $364.00 | |||
BTBD4 (C-5) Neutralizing Peptide | sc-390260 P | 100 µg/0.5 ml | $69.00 |