BTBD1 Antibody (A-12): sc-398235
- BTBD1 Antibody (A-12) is a mouse monoclonal IgG1 κ BTBD1 antibody provided at 200 µg/ml
- raised against amino acids 1-60 mapping at the N-terminus of BTBD1 of human origin
- BTBD1 Antibody (A-12) is recommended for detection of BTBD1 of human origin by WB, IP, IF and ELISA
- Anti-BTBD1 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BTBD1 Antibody (A-12) for WB applications. These reagents are now offered in bundles with BTBD1 Antibody (A-12) (see ordering information below).
BTBD1 Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects BTBD1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BTBD1 Antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BTBD1, also known as BTB/POZ domain containing 1 or NS5ATP8, is a cytoplasmic protein that plays a crucial role in myoblast growth and differentiation, making BTBD1 essential for muscle development and repair. BTBD1 is predominantly expressed in skeletal muscle and heart tissues, highlighting BTBD1′s importance in muscle function and regeneration. BTBD1 is widely conserved across species, with expression observed from C. elegans to humans, and BTBD1 specifically localizes to cytoplasmic bodies, which are thought to be involved in various cellular processes. BTBD1 features a BTB/POZ domain that facilitates protein-protein interactions, particularly with other transcription factors, and a C-terminal kelch-like region that allows BTBD1 to interact with Topoisomerase I, further underscoring BTBD1′s role in cellular regulation and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BTBD1 Antibody (A-12) References:
- Identification and characterization of BTBD1, a novel BTB domain containing gene on human chromosome 15q24. | Carim-Todd, L., et al. 2001. Gene. 262: 275-81. PMID: 11179693
- Characterization of BTBD1 and BTBD2, two similar BTB-domain-containing Kelch-like proteins that interact with Topoisomerase I. | Xu, L., et al. 2002. BMC Genomics. 3: 1. PMID: 11818025
- BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta. | Xu, L., et al. 2003. Exp Cell Res. 288: 84-93. PMID: 12878161
- The topoisomerase 1-interacting protein BTBD1 is essential for muscle cell differentiation. | Pisani, DF., et al. 2004. Cell Death Differ. 11: 1157-65. PMID: 15486563
- Thorough validation of siRNA-induced cell death phenotypes defines new anti-apoptotic protein. | Wu, W., et al. 2006. Nucleic Acids Res. 34: e13. PMID: 16432257
- Identification and isolation of a BTB-POZ-containing gene expressed in oocytes and early embryos of the zebrafish Danio rerio. | Smith, TH., et al. 2006. Genome. 49: 808-14. PMID: 16936789
- The role of BTB domain-containing zinc finger proteins in T cell development and function. | Bilic, I. and Ellmeier, W. 2007. Immunol Lett. 108: 1-9. PMID: 17084908
- Involvement of BTBD1 in mesenchymal differentiation. | Pisani, DF., et al. 2007. Exp Cell Res. 313: 2417-26. PMID: 17462629
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTBD1 Antibody (A-12) | sc-398235 | 200 µg/ml | $322.00 | |||
BTBD1 Antibody (A-12): m-IgG Fc BP-HRP Bundle | sc-530725 | 200 µg Ab; 10 µg BP | $361.00 | |||
BTBD1 Antibody (A-12): m-IgGκ BP-HRP Bundle | sc-524258 | 200 µg Ab, 40 µg BP | $361.00 | |||
BTBD1 Antibody (A-12): m-IgG1 BP-HRP Bundle | sc-544220 | 200 µg Ab; 20 µg BP | $361.00 | |||
BTBD1 Antibody (A-12) AC | sc-398235 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BTBD1 Antibody (A-12) HRP | sc-398235 HRP | 200 µg/ml | $322.00 | |||
BTBD1 Antibody (A-12) FITC | sc-398235 FITC | 200 µg/ml | $336.00 | |||
BTBD1 Antibody (A-12) PE | sc-398235 PE | 200 µg/ml | $349.00 | |||
BTBD1 Antibody (A-12) Alexa Fluor® 488 | sc-398235 AF488 | 200 µg/ml | $364.00 | |||
BTBD1 Antibody (A-12) Alexa Fluor® 546 | sc-398235 AF546 | 200 µg/ml | $364.00 | |||
BTBD1 Antibody (A-12) Alexa Fluor® 594 | sc-398235 AF594 | 200 µg/ml | $364.00 | |||
BTBD1 Antibody (A-12) Alexa Fluor® 647 | sc-398235 AF647 | 200 µg/ml | $364.00 | |||
BTBD1 Antibody (A-12) Alexa Fluor® 680 | sc-398235 AF680 | 200 µg/ml | $364.00 | |||
BTBD1 Antibody (A-12) Alexa Fluor® 790 | sc-398235 AF790 | 200 µg/ml | $364.00 |