SMARCA4/Brg1 Antibody (G-7): sc-17796

Brg-1 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Brg-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Brg-1 antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Brg-1, also known as SMARCA4, plays a crucial role in the SWI-SNF chromatin remodeling complex, which regulates gene expression through nucleosome structure remodeling in an ATP-dependent manner. This remodeling drives various cellular processes, including differentiation, proliferation, and response to external signals. Brg-1 interacts with other key SWI-SNF complex components, such as Brm (SNF2α), Ini1 (integrase interactor 1 or SNF5), BAF155, and BAF170, forming a functional core that enhances remodeling activity. These interacting proteins stabilize the complex and modulate activity, influencing the cell′s transcriptional landscape. Understanding SWI-SNF complex dynamics and components, including Brg-1, helps explain gene regulation mechanisms and their potential role in various diseases, including cancer.

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SMARCA4/Brg1 Antibody (G-7) References:

1. Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.  |  Phelan, ML., et al. 1999. Mol Cell. 3: 247-53. PMID: 10078207
2. BRG-1 is required for RB-mediated cell cycle arrest.  |  Strobeck, MW., et al. 2000. Proc Natl Acad Sci U S A. 97: 7748-53. PMID: 10884406
3. BRG-1 is recruited to estrogen-responsive promoters and cooperates with factors involved in histone acetylation.  |  DiRenzo, J., et al. 2000. Mol Cell Biol. 20: 7541-9. PMID: 11003650
4. Brg-1 is required for maximal transcription of the human matrix metalloproteinase-2 gene.  |  Ma, Z., et al. 2004. J Biol Chem. 279: 46326-34. PMID: 15317818
5. Brg-1 targeting of novel miR550a-5p/RNF43/Wnt signaling axis regulates colorectal cancer metastasis.  |  Wang, G., et al. 2016. Oncogene. 35: 651-61. PMID: 25961913
6. Brg-1 targeting of novel miR550a-5p/RNF43/Wnt signaling axis regulates colorectal cancer metastasis.  |  Wang, G., et al. 2017. Oncogene. 36: 5915. PMID: 28846106
7. Activation of Akt-dependent Nrf2/ARE pathway by restoration of Brg-1 remits high glucose-induced oxidative stress and ECM accumulation in podocytes.  |  Yan, H., et al. 2021. J Biochem Mol Toxicol. 35: e22672. PMID: 33270355
8. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor.  |  Muchardt, C. and Yaniv, M. 1993. EMBO J. 12: 4279-90. PMID: 8223438
9. BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription.  |  Khavari, PA., et al. 1993. Nature. 366: 170-4. PMID: 8232556
10. ISWI, a member of the SWI2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor.  |  Tsukiyama, T., et al. 1995. Cell. 83: 1021-6. PMID: 8521502
11. Nucleosome disruption by human SWI/SNF is maintained in the absence of continued ATP hydrolysis.  |  Imbalzano, AN., et al. 1996. J Biol Chem. 271: 20726-33. PMID: 8702824
12. Cloning and mapping of SMARCA5 encoding hSNF2H, a novel human homologue of Drosophila ISWI.  |  Aihara, T., et al. 1998. Cytogenet Cell Genet. 81: 191-3. PMID: 9730600

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMARCA4/Brg1 Antibody (G-7) has 240 citations in a variety of scientific publications.

Primary antibodies like Anti-SMARCA4/Brg1 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMARCA4/Brg1 Antibody (G-7) Specifications

Alternate Product Name: Brg1

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 209-296

Epitope Target: Brg-1

Epitope Species: human

Product Reactivity/Detection: Brg-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 240 citations in various scientific publications

Available Conjugates/Forms for SMARCA4/Brg1 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred SMARCA4/Brg1 antibody is SMARCA4/Brg1 Antibody (H-10): sc-374197.