BRE Antibody (D-2): sc-376453
- BRE Antibody (D-2) is a mouse monoclonal IgG1 κ BRE antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1-21 at the N-terminus of BRE of human origin
- BRE Antibody (D-2) is recommended for detection of BRE of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
- TransCruz reagent for ChIP application (sc-376453 X, 200 µg/0.1 ml)
- Anti-BRE Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for BRE Antibody (D-2). This work is in progress.
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SEE ALSO...
BRE Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects BRE protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BRE (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The brain and reproductive organ-expressed protein (BRE) is a 415 amino acid protein that plays a crucial role in regulating apoptosis by binding to the intracellular juxtamembrane domain of the death receptor tumor necrosis factor receptor 1 (TNF-R1) and the death receptor FAS. By downregulating TNFα-induced activation of NFκB, BRE contributes to cellular homeostasis and differentiation in epithelial, neural, and germ line cells. BRE′s ability to inhibit components of the death-inducing signaling complexes is vital for preventing unwanted apoptosis, making BRE an important player in cell survival. BRE is predominantly expressed in the adrenal cortex, medulla, testis, and pancreas, with lower expression levels in the thymus, thyroid, stomach, and small intestine. Additionally, BRE responds to various stimuli, including DNA-damaging agents in fibroblasts, lipopolysaccharides in peripheral blood mononuclear cells, and retinoic acid in brain glioma, highlighting BRE′s potential role in cellular responses to stress and differentiation signals.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BRE Antibody (D-2) References:
- A death receptor-associated anti-apoptotic protein, BRE, inhibits mitochondrial apoptotic pathway. | Li, Q., et al. 2004. J Biol Chem. 279: 52106-16. PMID: 15465831
- BRE enhances in vivo growth of tumor cells. | Chan, BC., et al. 2005. Biochem Biophys Res Commun. 326: 268-73. PMID: 15582573
- Blocking BRE expression in Leydig cells inhibits steroidogenesis by down-regulating 3beta-hydroxysteroid dehydrogenase. | Miao, J., et al. 2005. J Endocrinol. 185: 507-17. PMID: 15930177
- Identification of a brain- and reproductive-organs-specific gene responsive to DNA damage and retinoic acid. | Li, L., et al. 1995. Biochem Biophys Res Commun. 206: 764-74. PMID: 7826398
- BRE: a modulator of TNF-alpha action. | Gu, C., et al. 1998. FASEB J. 12: 1101-8. PMID: 9737713
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRE Antibody (D-2) | sc-376453 | 200 µg/ml | $322.00 | |||
BRE Antibody (D-2) X | sc-376453 X | 200 µg/0.1 ml | $322.00 | |||
BRE Antibody (D-2) AC | sc-376453 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BRE Antibody (D-2) HRP | sc-376453 HRP | 200 µg/ml | $322.00 | |||
BRE Antibody (D-2) FITC | sc-376453 FITC | 200 µg/ml | $336.00 | |||
BRE Antibody (D-2) PE | sc-376453 PE | 200 µg/ml | $349.00 | |||
BRE Antibody (D-2) Alexa Fluor® 488 | sc-376453 AF488 | 200 µg/ml | $364.00 | |||
BRE Antibody (D-2) Alexa Fluor® 546 | sc-376453 AF546 | 200 µg/ml | $364.00 | |||
BRE Antibody (D-2) Alexa Fluor® 594 | sc-376453 AF594 | 200 µg/ml | $364.00 | |||
BRE Antibody (D-2) Alexa Fluor® 647 | sc-376453 AF647 | 200 µg/ml | $364.00 | |||
BRE Antibody (D-2) Alexa Fluor® 680 | sc-376453 AF680 | 200 µg/ml | $364.00 | |||
BRE Antibody (D-2) Alexa Fluor® 790 | sc-376453 AF790 | 200 µg/ml | $364.00 | |||
BRE (D-2) Neutralizing Peptide | sc-376453 P | 100 µg/0.5 ml | $69.00 |