Date published: 2025-10-12

1-800-457-3801

SCBT Portrait Logo
Seach Input

BrdU Antibody (MoBU-1): sc-51514

5.0(6)
Write a reviewAsk a question

Datasheets
  • BrdU Antibody (MoBU-1) is a mouse monoclonal IgG1, cited in 25 publications, provided at 100 µg/ml
  • raised against 5-bromodeoxyuridine (BrdU) conjugated to hemocyanine
  • recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by IF, IHC(P) and FCM
  • recognizes BrdU in denatured DNA of cells labeled with BrdU
  • suggested companion product: 5-Bromo-2′ deoxyuridine: sc-290815
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BrdU Antibody (MoBU-1) for IHC(P) applications. These reagents are now offered in bundles with BrdU Antibody (MoBU-1) (see ordering information below).

QUICK LINKS

SEE ALSO...

BrdU Antibody (MoBU-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects BrdU in non-species reactive samples through immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-BrdU antibody (MoBU-1) is available in a non-conjugated form, specifically designed to identify bromodeoxyuridine, a thymidine analog incorporated into newly synthesized DNA during the S-phase of the cell cycle. This incorporation is crucial for assessing cell proliferation, as researchers can estimate the fraction of cells actively engaged in DNA synthesis. BrdU uptake quantification is particularly valuable in cancer biology studies, where understanding cell division rates can inform therapeutic strategies. BrdU (MoBU-1) monoclonal antibody is instrumental in investigating sister chromatid exchanges and isolating nascent DNA, providing insights into genomic stability and repair mechanisms. BrdU incorporation into DNA can be facilitated by UV-induced excision-repair synthesis, and anti-BrdU antibody (MoBU-1) binding occurs after a denaturation step, which exposes BrdU in single-stranded DNA. Protease antigen recovery may be necessary for optimal results in tissues or cells fixed with crosslinking agents like formalin, although this may affect BrdU immunodetection specificity.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

BrdU Antibody (MoBU-1)

sc-51514
100 µg/ml
$316.00

BrdU Antibody (MoBU-1): m-IgG Fc BP-HRP Bundle

sc-536846
100 µg Ab; 10 µg BP
$354.00

BrdU Antibody (MoBU-1): m-IgGκ BP-HRP Bundle

sc-534013
100 µg Ab; 40 µg BP
$354.00

Is it absolutely necessary to do a denaturing step to visualize the antibody by IF in paraffin sections? By treating the sections with 2M HCl, I am unable to see a proper DAPI staining and therefore cannot quantify the PERCENTAGE of BrdU positive cells.

Asked by: Mirabai
Thank you for your message. Please find on the following link our highly recommended protocol: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining If you have further questions, please do not hesitate to contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2021-08-17

What is the total volume provided of BrdU (MoBU-1): sc-51514 monoclonal antibody?

Asked by: cjMara
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2017-12-04
  • y_2025, m_10, d_9, h_7CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_51514, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 98ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Very Good IHC antibodyThis antibody consistently worked well for us on FFPE mouse small intestinal tissue.
Date published: 2020-06-27
Rated 5 out of 5 by from Reliable Antibody, Great for IHCThis antibody is great for producing images of dividing cells. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-18
Rated 5 out of 5 by from useful in scientific researchi bought BrdU antibody for my WB experiment ,it makes good result ,i recommand it
Date published: 2016-12-05
Rated 5 out of 5 by from IHC publishable data on rat sciatic nerveIHC publishable data on rat sciatic nerve - SCBT Publication Review
Date published: 2015-03-21
Rated 5 out of 5 by from Successfully used in Flow Cytometry withSuccessfully used in Flow Cytometry with human HCT116 cell - SCBT Publication Review
Date published: 2015-02-26
Rated 5 out of 5 by from Publishable IHC (P) data with mouse adrenalPublishable IHC (P) data with mouse adrenal glands - SCBT Publication Review
Date published: 2013-02-09
  • y_2025, m_10, d_9, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_51514, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
BrdU Antibody (MoBU-1) is rated 5.0 out of 5 by 6.
  • y_2025, m_10, d_9, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_51514, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 112ms
  • REVIEWS, PRODUCT