BRAP Antibody (D-5): sc-166012

BRAP Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects BRAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BRAP (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BRAP protein, also known as BRCA1 associated protein, RNF52, BRAP2, or IMP, is a 592 amino acid protein primarily localized in the cytoplasm and features one UBP-type zinc finger and one RING-type zinc finger. BRAP protein plays a crucial role in cellular signaling by negatively regulating MAP kinase activity, specifically through Ksr-1 scaffold protein inactivation, which limits Raf/MEK complex formation. MAP kinase pathway regulation is vital for controlling cell proliferation and differentiation, making BRAP an important player in cancer biology and other diseases. BRAP may also regulate nuclear transport and functions as a Ras-responsive E3 ubiquitin ligase, which undergoes auto-ubiquitination. BRAP gene is located on human chromosome 12, a region associated with various genetic disorders, including Noonan syndrome and Trisomy 12p, highlighting BRAP′s significance in both normal physiology and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BRAP Antibody (D-5) References:

1. Ras regulates assembly of mitogenic signalling complexes through the effector protein IMP.  |  Matheny, SA., et al. 2004. Nature. 427: 256-60. PMID: 14724641
2. Brap2 functions as a cytoplasmic retention protein for p21 during monocyte differentiation.  |  Asada, M., et al. 2004. Mol Cell Biol. 24: 8236-43. PMID: 15340083
3. IMP modulates KSR1-dependent multivalent complex formation to specify ERK1/2 pathway activation and response thresholds.  |  Chen, C., et al. 2008. J Biol Chem. 283: 12789-96. PMID: 18332145
4. Brap2 facilitates HsCdc14A Lys-63 linked ubiquitin modification.  |  Chen, JS., et al. 2009. Biotechnol Lett. 31: 615-21. PMID: 19152073
5. SNPs in BRAP associated with risk of myocardial infarction in Asian populations.  |  Ozaki, K., et al. 2009. Nat Genet. 41: 329-33. PMID: 19198608
6. The nuclear localization sequences of the BRCA1 protein interact with the importin-alpha subunit of the nuclear transport signal receptor.  |  Chen, CF., et al. 1996. J Biol Chem. 271: 32863-8. PMID: 8955125
7. Identification of a novel cytoplasmic protein that specifically binds to nuclear localization signal motifs.  |  Li, S., et al. 1998. J Biol Chem. 273: 6183-9. PMID: 9497340

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BRAP Antibody (D-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-BRAP Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BRAP Antibody (D-5) Specifications

Alternate Product Name: BRAP

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 41-340

Epitope Target: BRAP

Epitope Species: human

Product Reactivity/Detection: BRAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for BRAP Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin