BMP-4 Antibody (3H2.3) recommended for detection of presursor and mature BMP-4 of mouse, rat and human origin by WB, IP and IF

BMP-4 Antibody (3H2.3): sc-12721

BMP-4 Antibody (3H2.3) is rated 3.2 out of 5 by 10.
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Datasheets

    • BMP-4 Antibody (3H2.3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against recombinant BMP-4 of mouse origin
    • Anti-BMP-4 Antibody (3H2.3) is recommended for detection of presursor and mature BMP-4 of mouse, rat and human origin by WB, IP and IF
    • Anti-BMP-4 Antibody (3H2.3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BMP-4 (3H2.3): sc-12721.
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What is the molecular weight of mature BMP-4?

Asked by: AbPolly
The observed weight for the secreted mature form of BMP-4 is approximately 23 kDa.
Answered by: Technical Support 2
Date published: 2017-01-17
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Rated 1 out of 5 by from No band detected in WB for rat WAT No band detected in Western blot (1:100) for rat white adipose tissue.
Date published: 2018-09-12
Rated 1 out of 5 by from Did not work for BMMCs IF Tried 1:100 dilution using mouse BMMCs for IF and no signal detected
Date published: 2018-06-21
Rated 5 out of 5 by from Good Antibody BMP-4 (3H2.3) is well cited, over 20 product citations for recommended applications. Works well transfected lysates as positive control.
Date published: 2017-01-11
Rated 3 out of 5 by from Used on western blots at 1 g/mL with an Used on western blots at 1 g/mL with an overnight 4 C incubation and a protein-free blocking agent. This antibody cleanly detects 1 ng of recombinant human BMP4. However, in a side-by-side comparison the D-6 antibody (sc-393329) has a stronger signal and lower detection limit.
Date published: 2015-12-01
Rated 4 out of 5 by from Various publishable data in IHC(P) using Various publishable data in IHC(P) using mouse temporomandibular joint. -SCBT Publication Review
Date published: 2015-05-19
Rated 3 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-05-12
Rated 4 out of 5 by from Publishable data in IF using mouse embryos Publishable data in IF using mouse embryos. -SCBT Publication Review
Date published: 2015-04-15
Rated 4 out of 5 by from Various publishable data in WB using rat Various publishable data in WB using rat neural stem cell cultures and pulmonary artery smooth muscle . -SCBT Publication Review
Date published: 2015-03-24
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