BMP-4 Antibody (3H2.3): sc-12721

BMP-4 Antibody (3H2.3) is a mouse monoclonal IgG2b kappa light chain antibody that detects BMP-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). BMP-4 (3H2.3) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bone morphogenic proteins (BMPs), including BMP-4, are critical members of the transforming growth factor-beta (TGFβ) superfamily, playing essential roles in the regulation of bone and cartilage development. BMP-4 is particularly important due to involvement in the activation of the Smad signaling pathway, which is crucial for mediating the cellular responses to BMP signaling. Upon binding to its receptor, BMP-4 facilitates the phosphorylation of Smad1, leading to translocation into the nucleus where Smad1 regulates the expression of target genes involved in osteogenesis and chondrogenesis. This regulatory mechanism underscores BMP-4 significance in developmental processes and tissue homeostasis, as well as potential implications in bone-related disorders.

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BMP-4 Antibody (3H2.3) References:

1. Osteogenic protein-2. A new member of the transforming growth factor-beta superfamily expressed early in embryogenesis.  |  Ozkaynak, E., et al. 1992. J Biol Chem. 267: 25220-7. PMID: 1460021
2. The transforming growth factor-beta family.  |  Massagué, J. 1990. Annu Rev Cell Biol. 6: 597-641. PMID: 2177343
3. Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone.  |  Celeste, AJ., et al. 1990. Proc Natl Acad Sci U S A. 87: 9843-7. PMID: 2263636
4. Novel regulators of bone formation: molecular clones and activities.  |  Wozney, JM., et al. 1988. Science. 242: 1528-34. PMID: 3201241
5. BMP-4 Extraction from Extracellular Matrix and Analysis of Heparin-Binding Properties.  |  Aykul, S., et al. 2022. Mol Biotechnol. 64: 156-170. PMID: 34550550
6. Gremlin-1 and BMP-4 Overexpressed in Osteoarthritis Drive an Osteochondral-Remodeling Program in Osteoblasts and Hypertrophic Chondrocytes.  |  Pérez-Lozano, ML., et al. 2022. Int J Mol Sci. 23: PMID: 35216203
7. BMP-4 and BMP-7 Inhibit EMT in a Model of Anterior Subcapsular Cataract in Part by Regulating the Notch Signaling Pathway.  |  Jiang, F., et al. 2023. Invest Ophthalmol Vis Sci. 64: 12. PMID: 37043340
8. The Roles of GDF-9, BMP-15, BMP-4 and EMMPRIN in Folliculogenesis and In Vitro Fertilization.  |  Fountas, S., et al. 2024. J Clin Med. 13: PMID: 38999341
9. Recombinant Vgr-1/BMP-6-expressing tumors induce fibrosis and endochondral bone formation in vivo.  |  Gitelman, SE., et al. 1994. J Cell Biol. 126: 1595-609. PMID: 8089189
10. A human Mad protein acting as a BMP-regulated transcriptional activator.  |  Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
11. Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
12. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member.  |  McPherron, AC., et al. 1997. Nature. 387: 83-90. PMID: 9139826

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BMP-4 Antibody (3H2.3) has 39 citations in a variety of scientific publications.

Primary antibodies like Anti-BMP-4 Antibody (3H2.3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BMP-4 Antibody (3H2.3) Specifications

Alternate Product Name: BMP-4

Product Clone Name: 3H2.3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 293-408

Epitope Target: BMP-4

Epitope Species: mouse

Product Reactivity/Detection: presursor and mature BMP-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 39 citations in various scientific publications

Available Conjugates/Forms for BMP-4 Antibody (3H2.3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin