BMP-4 Antibody (3H2.3): sc-12721
- BMP-4 Antibody (3H2.3) is a mouse monoclonal IgG2b κ, cited in 39 publications, provided at 200 µg/ml
- raised against recombinant BMP-4 of mouse origin
- Anti-BMP-4 Antibody (3H2.3) is recommended for detection of presursor and mature BMP-4 of mouse, rat and human origin by WB, IP and IF
- Anti-BMP-4 Antibody (3H2.3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for BMP-4 Antibody (3H2.3) for WB applications. These reagents are now offered in bundles with BMP-4 Antibody (3H2.3) (see ordering information below).
BMP-4 Antibody (3H2.3) is a mouse monoclonal IgG2b kappa light chain antibody that detects BMP-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). BMP-4 (3H2.3) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bone morphogenic proteins (BMPs), including BMP-4, are critical members of the transforming growth factor-beta (TGFβ) superfamily, playing essential roles in the regulation of bone and cartilage development. BMP-4 is particularly important due to involvement in the activation of the Smad signaling pathway, which is crucial for mediating the cellular responses to BMP signaling. Upon binding to its receptor, BMP-4 facilitates the phosphorylation of Smad1, leading to translocation into the nucleus where Smad1 regulates the expression of target genes involved in osteogenesis and chondrogenesis. This regulatory mechanism underscores BMP-4 significance in developmental processes and tissue homeostasis, as well as potential implications in bone-related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BMP-4 Antibody (3H2.3) References:
- Osteogenic protein-2. A new member of the transforming growth factor-beta superfamily expressed early in embryogenesis. | Ozkaynak, E., et al. 1992. J Biol Chem. 267: 25220-7. PMID: 1460021
- The transforming growth factor-beta family. | Massagué, J. 1990. Annu Rev Cell Biol. 6: 597-641. PMID: 2177343
- Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. | Celeste, AJ., et al. 1990. Proc Natl Acad Sci U S A. 87: 9843-7. PMID: 2263636
- Novel regulators of bone formation: molecular clones and activities. | Wozney, JM., et al. 1988. Science. 242: 1528-34. PMID: 3201241
- BMP-4 Extraction from Extracellular Matrix and Analysis of Heparin-Binding Properties. | Aykul, S., et al. 2022. Mol Biotechnol. 64: 156-170. PMID: 34550550
- Gremlin-1 and BMP-4 Overexpressed in Osteoarthritis Drive an Osteochondral-Remodeling Program in Osteoblasts and Hypertrophic Chondrocytes. | Pérez-Lozano, ML., et al. 2022. Int J Mol Sci. 23: PMID: 35216203
- BMP-4 and BMP-7 Inhibit EMT in a Model of Anterior Subcapsular Cataract in Part by Regulating the Notch Signaling Pathway. | Jiang, F., et al. 2023. Invest Ophthalmol Vis Sci. 64: 12. PMID: 37043340
- The Roles of GDF-9, BMP-15, BMP-4 and EMMPRIN in Folliculogenesis and In Vitro Fertilization. | Fountas, S., et al. 2024. J Clin Med. 13: PMID: 38999341
- Recombinant Vgr-1/BMP-6-expressing tumors induce fibrosis and endochondral bone formation in vivo. | Gitelman, SE., et al. 1994. J Cell Biol. 126: 1595-609. PMID: 8089189
- A human Mad protein acting as a BMP-regulated transcriptional activator. | Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
- Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. | Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
- Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. | McPherron, AC., et al. 1997. Nature. 387: 83-90. PMID: 9139826
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-4 Antibody (3H2.3) | sc-12721 | 200 µg/ml | $322.00 | |||
BMP-4 Antibody (3H2.3): m-IgG Fc BP-HRP Bundle | sc-525459 | 200 µg Ab; 10 µg BP | $361.00 | |||
BMP-4 Antibody (3H2.3): m-IgG2b BP-HRP Bundle | sc-549563 | 200 µg Ab; 10 µg BP | $361.00 | |||
BMP-4 Antibody (3H2.3) AC | sc-12721 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BMP-4 Antibody (3H2.3) HRP | sc-12721 HRP | 200 µg/ml | $322.00 | |||
BMP-4 Antibody (3H2.3) FITC | sc-12721 FITC | 200 µg/ml | $336.00 | |||
BMP-4 Antibody (3H2.3) PE | sc-12721 PE | 200 µg/ml | $349.00 | |||
BMP-4 Antibody (3H2.3) Alexa Fluor® 488 | sc-12721 AF488 | 200 µg/ml | $364.00 | |||
BMP-4 Antibody (3H2.3) Alexa Fluor® 546 | sc-12721 AF546 | 200 µg/ml | $364.00 | |||
BMP-4 Antibody (3H2.3) Alexa Fluor® 594 | sc-12721 AF594 | 200 µg/ml | $364.00 | |||
BMP-4 Antibody (3H2.3) Alexa Fluor® 647 | sc-12721 AF647 | 200 µg/ml | $364.00 | |||
BMP-4 Antibody (3H2.3) Alexa Fluor® 680 | sc-12721 AF680 | 200 µg/ml | $364.00 | |||
BMP-4 Antibody (3H2.3) Alexa Fluor® 790 | sc-12721 AF790 | 200 µg/ml | $364.00 |