Bmi-1 Antibody (F-9): sc-390443

Bmi-1 Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bmi-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Bmi-1 (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bmi-1 protein plays a crucial role in cellular processes, particularly in gene expression regulation and stem cell properties maintenance. Bmi-1 is primarily located in the nucleus, where Bmi-1 functions as a transcriptional repressor, interacting with chromatin and influencing expression of genes involved in cell cycle regulation and development. Nuclear localization allows Bmi-1 to modulate transcription of target genes, including those associated with oncogenesis and stem cell maintenance. Bmi-1 has been implicated in repression of the Ink4a/Arf locus, which is essential for controlling cellular senescence and tumor suppression. Dysregulation of Bmi-1 expression can lead to enhanced proliferation and survival of cancer cells, highlighting Bmi-1′s importance in both normal development and cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bmi-1 Antibody (F-9) References:

1. The bmi-1 and mel-18 gene products define a new family of DNA-binding proteins involved in cell proliferation and tumorigenesis.  |  Goebl, MG. 1991. Cell. 66: 623. PMID: 1823128
2. Sequence similarity between the mammalian bmi-1 proto-oncogene and the Drosophila regulatory genes Psc and Su(z)2.  |  van Lohuizen, M., et al. 1991. Nature. 353: 353-5. PMID: 1922340
3. Expression of novel DNA-binding protein with zinc finger structure in various tumor cells.  |  Tagawa, M., et al. 1990. J Biol Chem. 265: 20021-6. PMID: 2246278
4. BMI-1 regulates DNA end resection and homologous recombination repair.  |  Fitieh, A., et al. 2022. Cell Rep. 38: 110536. PMID: 35320715
5. BMI-1 promotes breast cancer proliferation and metastasis through different mechanisms in different subtypes.  |  Liu, JY., et al. 2023. Cancer Sci. 114: 449-462. PMID: 36285479
6. Therapeutic inhibition of Bmi-1 ablates chemoresistant cancer stem cells in adenoid cystic carcinoma.  |  Sahara, S., et al. 2023. Oral Oncol. 142: 106437. PMID: 37267716
7. Bmi-1 promotes the proliferation, migration and invasion, and inhibits cell apoptosis of human retinoblastoma cells via RKIP.  |  Li, Q., et al. 2024. Sci Rep. 14: 14544. PMID: 38914697
8. Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new 'RING-finger' motif.  |  Ishida, A., et al. 1993. Gene. 129: 249-55. PMID: 8325509
9. mel-18, a Polycomb group-related mammalian gene, encodes a transcriptional negative regulator with tumor suppressive activity.  |  Kanno, M., et al. 1995. EMBO J. 14: 5672-8. PMID: 8521824
10. A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton.  |  Akasaka, T., et al. 1996. Development. 122: 1513-22. PMID: 8625838
11. Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex.  |  Alkema, MJ., et al. 1997. Genes Dev. 11: 226-40. PMID: 9009205
12. Mammalian Polycomb group genes are categorized as a new type of early response gene induced by B-cell receptor cross-linking.  |  Hasegawa, M., et al. 1998. Mol Immunol. 35: 559-63. PMID: 9809583

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bmi-1 Antibody (F-9) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-Bmi-1 Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bmi-1 Antibody (F-9) Specifications

Alternate Product Name: BMI1

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 228-326

Epitope Target: Bmi-1

Epitope Species: human

Product Reactivity/Detection: Bmi-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for Bmi-1 Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Bmi-1 antibody is Bmi-1 Antibody (1F4): sc-13519.