Bmi-1 Antibody (1F4): sc-13519

Bmi-1 Antibody (1F4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Bmi-1 in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Bmi-1 (1F4) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Bmi-1 plays a crucial role in regulating gene expression and is a key member of the Polycomb group proteins, which are essential for maintaining the repression of homeotic genes during embryonic development. Bmi-1 is primarily located in the nucleus, where Bmi-1 interacts with chromatin to modulate transcriptional activity. Nuclear localization of Bmi-1 is vital for function in gene silencing and cellular proliferation, as direct influence on chromatin structure and accessibility of target genes occurs. Notably, Bmi-1 has been implicated in the regulation of self-renewal and differentiation in stem cells, as well as in the development of various cancers, making Bmi-1 a significant focus of research in oncology and developmental biology. Interaction of Bmi-1 with other proteins, such as c-Myc and Pim, further underscores importance in oncogenic pathways and lymphomagenesis, highlighting the need for effective tools like Bmi-1 (1F4) antibody to study diverse roles in cellular processes.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bmi-1 Antibody (1F4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Bmi-1 Antibody (1F4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bmi-1 Antibody (1F4) Specifications

Alternate Product Name: Bmi-1

Product Clone Name: 1F4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Bmi-1

Epitope Species: Drosophila melanogaster

Product Reactivity/Detection: Bmi-1 of mouse and Drosophila origin, and PSC of Drosophila origin

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Drosophila

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Bmi-1 Antibody (1F4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Bmi-1 antibody is Bmi-1 Antibody (F-9): sc-390443.