Biglycan Antibody (3E2): sc-100857

Biglycan Antibody (3E2) is a mouse monoclonal IgG2a kappa light chain antibody that detects Biglycan protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Biglycan antibody (3E2) is available as the non-conjugated form. Biglycan, a class I small leucine-rich proteoglycan (SLRP) found in the extracellular matrix, plays a crucial role in regulating bone cell differentiation and proliferation, which is vital for maintaining skeletal integrity and function. Biglycan is primarily located in the extracellular matrix, where Biglycan interacts with various growth factors and cytokines, thereby influencing cellular signaling pathways that govern bone metabolism. Notably, Biglycan contains two chondroitin sulfate glucosaminoglycan (GAG) chains attached near its amino terminus, distinguishing Biglycan from the closely related SLRP, decorin, which has only one GAG chain. This structural difference is significant as Biglycan′s ability to bind to collagen and other matrix components ultimately impacts tissue organization and repair. Studies have shown that Biglycan-deficient specimens exhibit a reduced capacity to generate bone cell precursors, a diminished response to transforming growth factor beta, decreased collagen synthesis, and increased apoptosis. In clinical contexts, patients with rheumatoid arthritis demonstrate heightened immune responses to Biglycan, while those with osteoarthritis do not, indicating that variations in immune recognition of SLRPs may contribute to the pathogenesis of inflammatory rheumatic diseases.

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Biglycan Antibody (3E2) References:

1. Domains of apolipoprotein E involved in the binding to the protein core of biglycan of the vascular extracellular matrix: potential relationship between retention and anti-atherogenic properties of this apolipoprotein.  |  Klezovitch, O. and Scanu, AM. 2001. Trends Cardiovasc Med. 11: 263-8. PMID: 11709279
2. Biglycan is a repressor of amelogenin expression and enamel formation: an emerging hypothesis.  |  Goldberg, M., et al. 2002. J Dent Res. 81: 520-4. PMID: 12147740
3. Elevated levels of synovial fluid antibodies reactive with the small proteoglycans biglycan and decorin in patients with rheumatoid arthritis or other joint diseases.  |  Polgár, A., et al. 2003. Rheumatology (Oxford). 42: 522-7. PMID: 12649398
4. Biglycan knockout mice: new models for musculoskeletal diseases.  |  Young, MF., et al. 2002. Glycoconj J. 19: 257-62. PMID: 12975603
5. Differential roles for small leucine-rich proteoglycans in bone formation.  |  Waddington, RJ., et al. 2003. Eur Cell Mater. 6: 12-21; discussion 21. PMID: 14562268
6. Effects of interferon-gamma and tumor necrosis factor alpha on the expression of the genes encoding aggrecan, biglycan, and decorin core proteins in cultured human chondrocytes.  |  Dodge, GR., et al. 1998. Arthritis Rheum. 41: 274-83. PMID: 9485085

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Biglycan Antibody (3E2) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-Biglycan Antibody (3E2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Biglycan Antibody (3E2) Specifications

Alternate Product Name: Biglycan

Product Clone Name: 3E2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-369

Epitope Target: Biglycan

Epitope Species: human

Product Reactivity/Detection: precursor and mature Biglycan

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for Biglycan Antibody (3E2): N/A