BIG2 Antibody (H-6): sc-398042

BIG2 Antibody (H-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects BIG2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BIG2 (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BIG2 protein plays a crucial role in intracellular vesicular trafficking as a guanine nucleotide-exchange protein (GEP), which facilitates the exchange of GDP for GTP, thereby activating ADP-ribosylation factors (ARFs). These ARFs are essential for the regulation of membrane traffic and cytoskeletal dynamics, impacting processes such as endocytosis and exocytosis. BIG2 presence in macromolecular complexes that shuttle between the Golgi apparatus and the cytosol highlights BIG2′s importance in maintaining cellular organization and function. BIG2 interaction with protein kinase A (PKA) regulatory subunits suggests a potential role as an A kinase-anchoring protein (AKAP), which may coordinate the cAMP signaling pathway with ARF regulatory mechanisms, further emphasizing BIG2′s significance in cellular signaling and trafficking pathways.

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BIG2 Antibody (H-6) References:

1. Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors.  |  Togawa, A., et al. 1999. J Biol Chem. 274: 12308-15. PMID: 10212200
2. Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2).  |  Li, H., et al. 2003. Proc Natl Acad Sci U S A. 100: 1627-32. PMID: 12571360
3. Interaction of FK506-binding protein 13 with brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1): effects of FK506.  |  Padilla, PI., et al. 2003. Proc Natl Acad Sci U S A. 100: 2322-7. PMID: 12606707

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BIG2 Antibody (H-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-BIG2 Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BIG2 Antibody (H-6) Specifications

Alternate Product Name: BIG2

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 585-636

Epitope Target: BIG2

Epitope Species: human

Product Reactivity/Detection: BIG2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for BIG2 Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin