BIG1 Antibody (A-11): sc-376866
- BIG1 Antibody (A-11) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 285-419 mapping within an internal region of BIG1 of human origin
- recommended for detection of BIG1 of human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for BIG1 Antibody (A-11) for WB and IHC(P) applications. These reagents are now offered in bundles with BIG1 Antibody (A-11) (see ordering information below).
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BIG1 Antibody (A-11) is a mouse monoclonal IgG1 antibody that detects BIG1 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). BIG1 is a guanine nucleotide-exchange protein (GEP) that plays a crucial role in intracellular vesicular trafficking by facilitating the exchange of GDP for GTP, thereby activating ADP-ribosylation factors (ARFs). BIG1 (A-11) monoclonal antibody recognizes BIG1 primarily in the Golgi membranes and cytosol, where BIG1 exists in macromolecular complexes vital for regulatory functions. BIG1 contains a sec7 domain, which is integral to catalytic activity and inhibition by brefeldin A, a compound that disrupts Golgi structure. Anti-BIG1 antibody (A-11) targets a protein that functions as an A kinase-anchoring protein (AKAP) through association with protein kinase A (PKA) regulatory subunits, linking the cAMP signaling pathway with ARF regulation. This multifaceted role underscores BIG1′s importance in cellular processes and highlights its potential as a target for research into vesicular trafficking and related disorders.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BIG1 Antibody (A-11) | sc-376866 | 200 µg/ml | $322.00 | |||
BIG1 Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-540657 | 200 µg Ab; 10 µg BP | $361.00 | |||
BIG1 Antibody (A-11): m-IgG1 BP-HRP Bundle | sc-542275 | 200 µg Ab; 20 µg BP | $361.00 |