βENaC Antibody (D-3): sc-25354

βENaC Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects the beta ENaC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-βENaC antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The epithelial sodium channel (ENaC) plays a crucial role in sodium reabsorption across the apical surface of epithelial cells in the kidney, distal colon, lungs, and exocrine glands, which is vital for maintaining fluid and electrolyte balance in the body. ENaC is composed of four homologous subunits—α, β, γ, and δ—that can form various heterotetramers, with the most common configuration being two α subunits, one β subunit, and one γ subunit. The proper functioning of ENaC is essential for regulating sodium levels, and mutations in the PY motifs located at the carboxy terminus of the subunits can lead to significant health issues. For instance, gain-of-function mutations in these motifs are associated with Liddle′s syndrome, a genetic condition that causes hypertension due to excessive sodium reabsorption, while loss-of-function mutations can result in pseudohypoaldosteronism type I, characterized by salt wasting and low blood pressure. βENaC monoclonal antibody (D-3) is an invaluable tool for researchers studying the physiological and pathological roles of ENaC in various tissues and conditions.

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βENaC Antibody (D-3) References:

1. Aldosterone-mediated regulation of ENaC alpha, beta, and gamma subunit proteins in rat kidney.  |  Masilamani, S., et al. 1999. J Clin Invest. 104: R19-23. PMID: 10510339
2. Trafficking and cell surface stability of the epithelial Na+ channel expressed in epithelial Madin-Darby canine kidney cells.  |  Hanwell, D., et al. 2002. J Biol Chem. 277: 9772-9. PMID: 11773057
3. Liddle syndrome: genetics and mechanisms of Na+ channel defects.  |  Warnock, DG. 2001. Am J Med Sci. 322: 302-7. PMID: 11780687
4. Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits.  |  Brockway, LM., et al. 2002. Am J Physiol Cell Physiol. 283: C126-34. PMID: 12055080
5. Severe hyperkalemia is rescued by low-potassium diet in renal βENaC-deficient mice.  |  Boscardin, E., et al. 2017. Pflugers Arch. 469: 1387-1399. PMID: 28567665
6. The SPLUNC1-βENaC complex prevents Burkholderia cenocepacia invasion in normal airway epithelia.  |  Ahmad, S., et al. 2020. Respir Res. 21: 190. PMID: 32680508
7. βENaC and ASIC2 associate in VSMCs to mediate pressure-induced constriction in the renal afferent arteriole.  |  Lu, Y., et al. 2022. Am J Physiol Renal Physiol. 322: F498-F511. PMID: 35285274
8. Inhalation of nicotine-containing electronic cigarette vapor exacerbates the features of COPD by inducing ferroptosis in βENaC-overexpressing mice.  |  Han, H., et al. 2024. Front Immunol. 15: 1429946. PMID: 38947318
9. Mice lacking ASIC2 and βENaC are protected from high-fat-diet-induced metabolic syndrome.  |  Hamby, M., et al. 2024. Front Endocrinol (Lausanne). 15: 1449344. PMID: 39224121
10. Cloning, chromosomal localization, and physical linkage of the beta and gamma subunits (SCNN1B and SCNN1G) of the human epithelial amiloride-sensitive sodium channel.  |  Voilley, N., et al. 1995. Genomics. 28: 560-5. PMID: 7490094
11. Cloning, expression, and tissue distribution of a human amiloride-sensitive Na+ channel.  |  McDonald, FJ., et al. 1994. Am J Physiol. 266: L728-34. PMID: 8023962
12. Structural organisation of the gene encoding the alpha-subunit of the human amiloride-sensitive epithelial sodium channel.  |  Ludwig, M., et al. 1998. Hum Genet. 102: 576-81. PMID: 9654208

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-βENaC Antibody (D-3) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-βENaC Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

βENaC Antibody (D-3) Specifications

Alternate Product Name: SCNN1B

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 271-460

Epitope Target: βENaC

Epitope Species: human

Product Reactivity/Detection: βENaC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for βENaC Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred βENaC antibody is βENaC Antibody (E-10): sc-48428.