beta ENaC Antibody (D-3) recommended for detection of βENaC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

βENaC Antibody (D-3): sc-25354

βENaC Antibody (D-3) is rated 5.0 out of 5 by 4.
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Datasheets

    • βENaC Antibody (D-3) is a mouse monoclonal IgG1 (kappa light chain) βENaC antibody provided at 200 µg/ml
    • raised against amino acids 271-460 of βENaC of human origin
    • beta ENaC Antibody (D-3) is recommended for detection of βENaC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • beta ENaC Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of βENaC (D-3): sc-25354.
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Can βENaC (D-3): sc-25354 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, βENaC (D-3): sc-25354 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good WB data with Mouse liver I bought it one month,used it 1:1000 in Mouse liver,obtained wonderful clean bands
Date published: 2017-01-07
Rated 5 out of 5 by from Published data for WB in transfected mCCD Published data for WB in transfected mCCD (mouse) cells. -SCBT Publication Review
Date published: 2015-05-29
Rated 5 out of 5 by from Good specificity in WB in KNRK whole cell lysate Good specificity in WB in KNRK whole cell lysate. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and nuclear Superb immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-04-30
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