βENaC Antibody (E-10): sc-48428

βENaC Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects βENaC in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-βENaC antibody (E-10) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. The epithelial sodium channel (ENaC), which βENaC targets, plays a crucial role in sodium reabsorption across epithelial tissues, particularly in the kidney, distal colon, lungs, and exocrine glands. This channel is composed of four homologous subunits—α, β, γ, and δ—that form a heterotetramer, with the most common configuration being two α subunits, one β, and one γ. Proper functioning of ENaC is vital for maintaining sodium balance and blood pressure regulation; dysregulation can lead to conditions such as Liddle′s syndrome, characterized by hypertension due to excessive sodium absorption, or pseudohypoaldosteronism type I, which results in salt wasting and low blood pressure. The αENaC gene is located on human chromosome 12p13, while the β and γENaC genes are found on chromosome 16p12, with both the β and γ subunits being glycosylated. The carboxy terminus of all ENaC subunits contains proline-tyrosine (PY) motifs that interact with the ubiquitin protein ligase Nedd4, which is essential for regulating intracellular sodium levels. Understanding the structure and function of ENaC is critical for elucidating its role in various physiological and pathological processes, making βENaC monoclonal antibody (E-10) an invaluable tool for researchers studying sodium transport and related disorders.

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βENaC Antibody (E-10) References:

1. Pressure-induced constriction is inhibited in a mouse model of reduced betaENaC.  |  VanLandingham, LG., et al. 2009. Am J Physiol Regul Integr Comp Physiol. 297: R723-8. PMID: 19553501
2. Altered whole kidney blood flow autoregulation in a mouse model of reduced beta-ENaC.  |  Grifoni, SC., et al. 2010. Am J Physiol Renal Physiol. 298: F285-92. PMID: 19889952
3. Airway surface liquid volume regulation determines different airway phenotypes in liddle compared with betaENaC-overexpressing mice.  |  Mall, MA., et al. 2010. J Biol Chem. 285: 26945-26955. PMID: 20566636
4. Morphological analysis of the trachea and pattern of breathing in βENaC-Tg mice.  |  Bonora, M., et al. 2011. Respir Physiol Neurobiol. 178: 346-8. PMID: 21700000
5. Transgenic hCFTR expression fails to correct β-ENaC mouse lung disease.  |  Grubb, BR., et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302: L238-47. PMID: 22003093
6. Impaired myogenic constriction of the renal afferent arteriole in a mouse model of reduced βENaC expression.  |  Ge, Y., et al. 2012. Am J Physiol Renal Physiol. 302: F1486-93. PMID: 22419697
7. CFTR regulates early pathogenesis of chronic obstructive lung disease in βENaC-overexpressing mice.  |  Johannesson, B., et al. 2012. PLoS One. 7: e44059. PMID: 22937152
8. Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells.  |  Duncan, JW., et al. 2020. Int J Mol Sci. 21: PMID: 32331392
9. Therapeutic Inhibition of Cathepsin S Reduces Inflammation and Mucus Plugging in Adult βENaC-Tg Mice.  |  Brown, R., et al. 2021. Mediators Inflamm. 2021: 6682657. PMID: 33828414
10. Bone marrow monocytes and macrophages from mice lacking βENaC and ASIC2 have a reduced chemotactic migration response and polarization.  |  Wasson, R., et al. 2024. Physiol Rep. 12: e16139. PMID: 39016176

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-βENaC Antibody (E-10) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-βENaC Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

βENaC Antibody (E-10) Specifications

Alternate Product Name: βENaC

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 271-460

Epitope Target: βENaC

Epitope Species: human

Product Reactivity/Detection: βENaC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for βENaC Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred βENaC antibody is βENaC Antibody (D-3): sc-25354.