beta ENaC Antibody (E-10) recommended for detection of βENaC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

βENaC Antibody (E-10): sc-48428

βENaC Antibody (E-10) is rated 5.0 out of 5 by 5.
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Datasheets

    • βENaC Antibody (E-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 271-460 of βENaC of human origin
    • beta ENaC Antibody (E-10) is recommended for detection of βENaC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • beta ENaC Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of βENaC (E-10): sc-48428.
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I am using βENaC (E-10): sc-48428 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced publishable WB data in mouse and rat This beta eNaC monoclonal is referenced in the literature for the use of WB in mouse kidney and rat KNRK cells.
Date published: 2017-01-24
Rated 5 out of 5 by from Published data for WB in human cystic fibrosis Published data for WB in human cystic fibrosis bronchial epithelial(CFBE) cell. -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by from WB publishable data with human cystic fibrosis WB publishable data with human cystic fibrosis bronchial epithelial(CFBE) cell - SCBT Publication Review
Date published: 2015-02-17
Rated 5 out of 5 by from Good specificity in WB in KNRK whole cell lysate Good specificity in WB in KNRK whole cell lysate. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human upper stomach tissue. -SCBT QC
Date published: 2013-12-18
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