beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (C-5): sc-515763

β3-AR Antibody (C-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects beta 3-AR protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). β3-AR Antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The beta 3-adrenergic receptor (β3-AR) plays a crucial role in regulating lipolysis and thermogenesis in adipose tissue, making β3-AR an important target for research in metabolic disorders and obesity. Located primarily in adipose tissues, the heart, and the smooth muscle of the bladder, colon, small intestine, and stomach, β3-AR is essential for maintaining energy homeostasis and cardiovascular function. Notably, in the human corpus cavernosum, β3-AR contributes to vasorelaxation, which is linked to the inhibition of the RhoA/Rho-kinase pathway, highlighting β3-AR significance in vascular health. Furthermore, β3-AR interacts directly with the SH3 domain of Src through proline-rich motifs (PXXP) found in the third intracellular loop and the carboxy-terminus, indicating β3-AR involvement in intracellular signaling pathways that regulate various physiological processes.

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beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (C-5) References:

1. Expression of human (beta)3-adrenergic receptor induces adipocyte-like features in CHO/K1 fibroblasts.  |  Gros, J., et al. 1999. J Cell Sci. 112 (Pt 21): 3791-7. PMID: 10523514
2. Direct binding of activated c-Src to the beta 3-adrenergic receptor is required for MAP kinase activation.  |  Cao, W., et al. 2000. J Biol Chem. 275: 38131-4. PMID: 11013230
3. CCAAT/enhancer-binding protein alpha is required for transcription of the beta 3-adrenergic receptor gene during adipogenesis.  |  Dixon, TM., et al. 2001. J Biol Chem. 276: 722-8. PMID: 11024036
4. Beta 3-adrenergic receptors regulate retinal endothelial cell migration and proliferation.  |  Steinle, JJ., et al. 2003. J Biol Chem. 278: 20681-6. PMID: 12670949
5. Involvement of beta 3-adrenergic receptor activation via cyclic GMP- but not NO-dependent mechanisms in human corpus cavernosum function.  |  Cirino, G., et al. 2003. Proc Natl Acad Sci U S A. 100: 5531-6. PMID: 12707413
6. beta(3) Adrenergic receptor polymorphism and obesity-related phenotypes in hypertensive patients.  |  Hao, K., et al. 2004. Obes Res. 12: 125-30. PMID: 14742851
7. Cardioprotective Effects of Beta3-Adrenergic Receptor (β3-AR) Pre-, Per-, and Post-treatment in Ischemia-Reperfusion.  |  Salie, R., et al. 2019. Cardiovasc Drugs Ther. 33: 163-177. PMID: 30729348
8. Corylin reduces obesity and insulin resistance and promotes adipose tissue browning through SIRT-1 and β3-AR activation.  |  Chen, CC., et al. 2021. Pharmacol Res. 164: 105291. PMID: 33253817
9. Comparative Transcriptome Profiling of Cold Exposure and β3-AR Agonist CL316,243-Induced Browning of White Fat.  |  Li, Y., et al. 2021. Front Physiol. 12: 667698. PMID: 34017267
10. β3AR-Dependent Brain-Derived Neurotrophic Factor (BDNF) Generation Limits Chronic Postischemic Heart Failure.  |  Cannavo, A., et al. 2023. Circ Res. 132: 867-881. PMID: 36884028
11. Chronic β3-AR stimulation activates distinct thermogenic mechanisms in brown and white adipose tissue and improves systemic metabolism in aged mice.  |  Natarajan, D., et al. 2024. Aging Cell. 23: e14321. PMID: 39177077
12. Obesity and diabetes and the beta-3 adrenergic receptor.  |  Danforth, E. and Himms-Hagen, JH. 1997. Eur J Endocrinol. 136: 362-5. PMID: 9150693

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (C-5) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (C-5) Specifications

Alternate Product Name: Beta-3 Adrenergic Receptor

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 351-400

Epitope Target: β₃-AR

Epitope Species: mouse

Product Reactivity/Detection: β₃-AR

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred beta 3 Adrenergic Receptor/ADRB3/β3-AR antibody is beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (B-12): sc-518080.